257674

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 61480..62123 Replicon plasmid pHB42-F3
Accession NZ_CP104331
Organism Escherichia coli strain THB42-F3

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag N0544_RS22685 Protein ID WP_001044768.1
Coordinates 61480..61896 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag N0544_RS22690 Protein ID WP_001261287.1
Coordinates 61893..62123 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0544_RS22670 (57979) 57979..58569 - 591 WP_000194575.1 hypothetical protein -
N0544_RS22675 (58569) 58569..58826 - 258 WP_000343085.1 hypothetical protein -
N0544_RS22680 (59180) 59180..61318 + 2139 WP_000350638.1 AAA family ATPase -
N0544_RS22685 (61480) 61480..61896 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
N0544_RS22690 (61893) 61893..62123 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
N0544_RS22695 (62419) 62419..62709 + 291 WP_000111771.1 hypothetical protein -
N0544_RS22700 (62699) 62699..63598 + 900 WP_000963206.1 nucleotide-binding protein -
N0544_RS22705 (63648) 63648..65873 - 2226 WP_000698737.1 P-loop NTPase fold protein -
N0544_RS22710 (65875) 65875..66963 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / mph(A) / aph(3')-IIa / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / blaTEM-1B / rmtB / blaCTX-M-55 - 1..161931 161931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T257674 WP_001044768.1 NZ_CP104331:c61896-61480 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT257674 WP_001261287.1 NZ_CP104331:c62123-61893 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References