Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4131779..4132276 Replicon chromosome
Accession NZ_CP104330
Organism Escherichia coli strain THB42-F3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N0544_RS20200 Protein ID WP_001173343.1
Coordinates 4131779..4131952 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N0544_RS20205 Protein ID WP_000398619.1
Coordinates 4131980..4132276 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0544_RS20190 (4128449) 4128449..4129906 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
N0544_RS20195 (4130143) 4130143..4131660 + 1518 WP_001295074.1 lysine--tRNA ligase -
N0544_RS20200 (4131779) 4131779..4131952 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N0544_RS20205 (4131980) 4131980..4132276 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N0544_RS20210 (4132503) 4132503..4132775 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
N0544_RS20215 (4132787) 4132787..4133017 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N0544_RS20220 (4133198) 4133198..4134829 + 1632 WP_001216477.1 sensor histidine kinase -
N0544_RS20225 (4134826) 4134826..4135545 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T257671 WP_001173343.1 NZ_CP104330:c4131952-4131779 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT257671 WP_000398619.1 NZ_CP104330:c4132276-4131980 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References