Detailed information of TA system TA025767

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PrlF(antitoxin)
• Location: 1320464..1321028
• Replicon: chromosome
• Accession: NC_016622
• Organism: Azospirillum lipoferum 4B

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AZOLI_RS06060
• Protein ID: WP_044549784.1
• Coordinates: 1320464..1320826 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G7Z703
• Locus tag: AZOLI_RS06065
• Protein ID: WP_014247717.1
• Coordinates: 1320813..1321028 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZOLI_RS06035	1315538..1316392	-	855	WP_014247711.1	squalene/phytoene synthase family protein	-
AZOLI_RS06040	1316517..1317389	+	873	WP_014247712.1	squalene/phytoene synthase family protein	-
AZOLI_RS06045	1317451..1318398	+	948	WP_162487999.1	presqualene diphosphate synthase HpnD	-
AZOLI_RS06050	1318399..1319799	+	1401	WP_014247714.1	hydroxysqualene dehydroxylase HpnE	-
AZOLI_RS06055	1319806..1320399	+	594	WP_014247715.1	superoxide dismutase	-
AZOLI_RS06060	1320464..1320826	-	363	WP_044549784.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AZOLI_RS06065	1320813..1321028	-	216	WP_014247717.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
AZOLI_RS06070	1321091..1321468	-	378	WP_014247718.1	Mth938-like domain-containing protein	-
AZOLI_RS06075	1321500..1322456	-	957	WP_014247719.1	protein translocase subunit SecF	-
AZOLI_RS06080	1322468..1324066	-	1599	WP_014247720.1	protein translocase subunit SecD	-
AZOLI_RS06085	1324193..1324624	-	432	WP_014247721.1	preprotein translocase subunit YajC	-
AZOLI_RS06090	1324850..1325746	+	897	WP_014247722.1	ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-104)

Antitoxin

• PrlF antitoxin (4-52)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13045.01 Da
• Isoelectric Point: 5.6977

>T25767 WP_044549784.1 NC_016622:c1320826-1320464 [Azospirillum lipoferum 4B]
MPIFETWNVVKVPFPYADRPVRQRRPALVVAADMLETSHSLIWVLMITSAENRGWPSDVPVSDIDTAGLPVPSVVRTAKV
AVIDARDAELLGSLVDSDRRAISAQVGGHLAEMLKAGRDS

Nucleotide

• Length: 363 bp

>T25767 NC_016622:c1320826-1320464 [Azospirillum lipoferum 4B]
ATGCCGATCTTTGAAACCTGGAACGTCGTCAAGGTTCCGTTCCCTTACGCCGATCGCCCGGTCAGGCAGCGCCGACCGGC
ATTGGTCGTTGCAGCTGACATGCTCGAAACAAGCCACAGCCTGATCTGGGTGTTGATGATTACCAGCGCCGAGAACAGGG
GGTGGCCTTCGGATGTACCTGTCAGTGACATCGATACGGCCGGGTTGCCGGTCCCATCGGTCGTGCGCACGGCCAAAGTC
GCTGTGATCGATGCGCGTGACGCCGAACTGCTTGGTTCGCTTGTTGACAGTGATCGTCGGGCGATCAGCGCGCAGGTTGG
CGGGCATCTTGCCGAAATGCTGAAAGCCGGCAGGGACAGCTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7987.01 Da
• Isoelectric Point: 4.7005

>AT25767 WP_014247717.1 NC_016622:c1321028-1320813 [Azospirillum lipoferum 4B]
MITSKLTSKAQTTIPQAVRTALGLHEGDEIAYRIEDGRVILTRVAPVPVDDPFHCFTEWDSEVDRRAYADL

Nucleotide

• Length: 216 bp

>AT25767 NC_016622:c1321028-1320813 [Azospirillum lipoferum 4B]
ATGATCACCAGCAAACTGACCAGTAAAGCTCAGACGACGATTCCGCAGGCAGTGCGGACTGCGCTGGGCCTTCATGAGGG
CGACGAGATCGCCTACCGCATTGAAGACGGCCGCGTCATCCTGACACGGGTCGCGCCGGTTCCTGTCGACGACCCCTTCC
ATTGCTTCACGGAATGGGACAGCGAAGTCGACCGGCGGGCCTATGCCGATCTTTGA

Structures

Antitoxin

• AlphaFold DB: G7Z703