257664

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3523390..3524069 Replicon chromosome
Accession NZ_CP104330
Organism Escherichia coli strain THB42-F3

Toxin (Protein)


Gene name ykfI Uniprot ID P77692
Locus tag N0544_RS17175 Protein ID WP_000854672.1
Coordinates 3523390..3523731 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID Q47684
Locus tag N0544_RS17180 Protein ID WP_000070395.1
Coordinates 3523752..3524069 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0544_RS17150 (3518976) 3518976..3519068 + 93 Protein_3350 sigma factor-binding protein Crl -
N0544_RS17155 (3519107) 3519107..3520162 - 1056 WP_000749863.1 phosphoporin PhoE -
N0544_RS17160 (3520450) 3520450..3521553 + 1104 WP_001285288.1 glutamate 5-kinase -
N0544_RS17165 (3521565) 3521565..3522818 + 1254 WP_000893278.1 glutamate-5-semialdehyde dehydrogenase -
N0544_RS17175 (3523390) 3523390..3523731 - 342 WP_000854672.1 type IV toxin-antitoxin system toxin YkfI Toxin
N0544_RS17180 (3523752) 3523752..3524069 - 318 WP_000070395.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
N0544_RS17185 (3524088) 3524088..3524309 - 222 WP_000691994.1 DUF987 domain-containing protein -
N0544_RS17190 (3524318) 3524318..3524794 - 477 WP_000811693.1 RadC family protein -
N0544_RS17195 (3524810) 3524810..3525268 - 459 WP_000211838.1 antirestriction protein -
N0544_RS17200 (3525366) 3525366..3525605 - 240 WP_000194654.1 DUF905 family protein -
N0544_RS17205 (3525682) 3525682..3526149 - 468 WP_001547765.1 protein YkfB -
N0544_RS17210 (3526172) 3526172..3526615 - 444 WP_000824223.1 lipoprotein YafY -
N0544_RS17215 (3526615) 3526615..3526791 - 177 WP_001285112.1 hypothetical protein -
N0544_RS17220 (3526838) 3526838..3527029 - 192 Protein_3363 DeoR family transcriptional regulator -
N0544_RS17225 (3527246) 3527246..3528067 - 822 WP_000197389.1 DUF932 domain-containing protein -
N0544_RS17230 (3528159) 3528159..3529022 - 864 WP_001065553.1 GTPase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12905.01 Da        Isoelectric Point: 9.6543

>T257664 WP_000854672.1 NZ_CP104330:c3523731-3523390 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11939.59 Da        Isoelectric Point: 6.7391

>AT257664 WP_000070395.1 NZ_CP104330:c3524069-3523752 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P77692

Antitoxin

Source ID Structure
AlphaFold DB Q47684

References