Detailed information of TA system TA025766

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 744755..745357
• Replicon: chromosome
• Accession: NC_016622
• Organism: Azospirillum lipoferum 4B

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G7Z9L1
• Locus tag: AZOLI_RS03400
• Protein ID: WP_014247178.1
• Coordinates: 744755..745045 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G7Z9L2
• Locus tag: AZOLI_RS03405
• Protein ID: WP_014247179.1
• Coordinates: 745073..745357 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZOLI_RS03385	740218..742269	+	2052	WP_014247176.1	molybdopterin oxidoreductase family protein	-
AZOLI_RS03390	742551..744347	+	1797	WP_014247177.1	single-stranded-DNA-specific exonuclease RecJ	-
AZOLI_RS03400	744755..745045	+	291	WP_014247178.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AZOLI_RS03405	745073..745357	+	285	WP_014247179.1	putative addiction module antidote protein	Antitoxin
AZOLI_RS03410	745411..746418	+	1008	WP_014247180.1	tRNA dihydrouridine(20/20a) synthase DusA	-
AZOLI_RS03415	746464..748566	-	2103	WP_014247181.1	HAMP domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-93)

Antitoxin

• Uncharacterized protein HI1420 (6-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10927.51 Da
• Isoelectric Point: 9.6893

>T25766 WP_014247178.1 NC_016622:744755-745045 [Azospirillum lipoferum 4B]
MIEIRQTDRFAAWIESLRDDRARMRIEVRIRRLQLGNPGDTKPVGEGVSELRIDYGPGYRVYFIQRGDTLIVLLAGGDKR
SQDRDIAAAIALARDL

Nucleotide

• Length: 291 bp

>T25766 NC_016622:744755-745045 [Azospirillum lipoferum 4B]
ATGATCGAGATCCGCCAGACCGACCGCTTCGCCGCTTGGATAGAGAGCTTGCGGGATGATCGGGCTCGCATGCGGATCGA
AGTGCGCATCCGCCGGCTTCAGCTCGGCAATCCCGGCGACACGAAGCCGGTGGGCGAGGGGGTCAGCGAACTGCGCATCG
ATTACGGCCCCGGTTACCGCGTCTATTTCATCCAGCGCGGCGACACGCTGATCGTGCTGCTGGCCGGCGGAGACAAGCGG
TCCCAGGACCGCGACATCGCCGCCGCCATCGCGCTGGCGCGGGACCTCTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10081.49 Da
• Isoelectric Point: 4.5600

>AT25766 WP_014247179.1 NC_016622:745073-745357 [Azospirillum lipoferum 4B]
MGRTETRPWDIVDSLDSDERIAAYLDAVLEDGDPQLIVAALGDIARAKGMTEVARNAGLGRQSLYKALSPDGNPEFATVL
KVIKSLGLRLTVSP

Nucleotide

• Length: 285 bp

>AT25766 NC_016622:745073-745357 [Azospirillum lipoferum 4B]
ATGGGCCGAACCGAGACCCGTCCCTGGGACATCGTCGACAGCCTGGACAGTGACGAGCGCATCGCCGCCTATCTGGATGC
GGTGCTGGAGGATGGCGATCCGCAACTGATCGTCGCGGCGCTCGGCGACATCGCCCGCGCAAAGGGGATGACGGAGGTCG
CTCGCAACGCCGGGCTCGGCCGCCAAAGCCTCTACAAGGCGCTGTCACCGGACGGCAACCCGGAATTCGCCACTGTCCTG
AAGGTGATCAAGTCTCTCGGACTTCGCCTGACCGTCAGTCCATAG

Structures

Toxin

• AlphaFold DB: G7Z9L1

Antitoxin

• AlphaFold DB: G7Z9L2