Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 41106..41749 Replicon plasmid pHB42-F2
Accession NZ_CP104329
Organism Escherichia coli strain THB42-F2

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag N0499_RS22585 Protein ID WP_001044768.1
Coordinates 41106..41522 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag N0499_RS22590 Protein ID WP_001261287.1
Coordinates 41519..41749 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0499_RS22570 (N0499_22570) 37605..38195 - 591 WP_000194575.1 hypothetical protein -
N0499_RS22575 (N0499_22575) 38195..38452 - 258 WP_000343085.1 hypothetical protein -
N0499_RS22580 (N0499_22580) 38806..40944 + 2139 WP_000350638.1 AAA family ATPase -
N0499_RS22585 (N0499_22585) 41106..41522 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
N0499_RS22590 (N0499_22590) 41519..41749 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
N0499_RS22595 (N0499_22595) 42045..42335 + 291 WP_000111771.1 hypothetical protein -
N0499_RS22600 (N0499_22600) 42325..43224 + 900 WP_000963206.1 nucleotide-binding protein -
N0499_RS22605 (N0499_22605) 43274..45499 - 2226 WP_000698737.1 P-loop NTPase fold protein -
N0499_RS22610 (N0499_22610) 45501..46589 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / aph(3')-IIa / mcr-1.1 - 1..123517 123517


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T257643 WP_001044768.1 NZ_CP104329:c41522-41106 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT257643 WP_001261287.1 NZ_CP104329:c41749-41519 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References