Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 5313168..5313758 Replicon chromosome
Accession NC_016612
Organism Klebsiella oxytoca KCTC 1686

Toxin (Protein)


Gene name pemK Uniprot ID A0A0H3HDW1
Locus tag KOX_RS24515 Protein ID WP_014229979.1
Coordinates 5313168..5313500 (-) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag KOX_RS24520 Protein ID WP_004852307.1
Coordinates 5313501..5313758 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KOX_RS24505 5309195..5311651 - 2457 WP_014229977.1 DEAD/DEAH box helicase -
KOX_RS24510 5311648..5312454 - 807 WP_064387424.1 hypothetical protein -
KOX_RS24515 5313168..5313500 - 333 WP_014229979.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KOX_RS24520 5313501..5313758 - 258 WP_004852307.1 antitoxin Antitoxin
KOX_RS29435 5314469..5314549 + 81 Protein_4862 molybdopterin-binding protein -
KOX_RS24530 5314740..5315654 - 915 WP_014229981.1 LysR family transcriptional regulator -
KOX_RS24535 5315755..5316753 + 999 WP_014229982.1 aldo/keto reductase -
KOX_RS24540 5316791..5317717 - 927 WP_014229983.1 LysR family transcriptional regulator -
KOX_RS24545 5317894..5318502 + 609 WP_014229984.1 glutathione S-transferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11740.57 Da        Isoelectric Point: 10.1863

>T25764 WP_014229979.1 NC_016612:c5313500-5313168 [Klebsiella michiganensis KCTC 1686]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGTKTTGVIRCDQPRTI
DMAARNGKRLESIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T25764 NC_016612:c5313500-5313168 [Klebsiella michiganensis KCTC 1686]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGCAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT25764 WP_004852307.1 NC_016612:c5313758-5313501 [Klebsiella michiganensis KCTC 1686]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT25764 NC_016612:c5313758-5313501 [Klebsiella michiganensis KCTC 1686]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3HDW1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References