Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 64169..64812 Replicon plasmid pHB42-F1
Accession NZ_CP104327
Organism Escherichia coli strain THB42-F1

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag N0470_RS22915 Protein ID WP_001044768.1
Coordinates 64169..64585 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag N0470_RS22920 Protein ID WP_001261287.1
Coordinates 64582..64812 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0470_RS22900 (60668) 60668..61258 - 591 WP_000194575.1 hypothetical protein -
N0470_RS22905 (61258) 61258..61515 - 258 WP_000343085.1 hypothetical protein -
N0470_RS22910 (61869) 61869..64007 + 2139 WP_000350638.1 AAA family ATPase -
N0470_RS22915 (64169) 64169..64585 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
N0470_RS22920 (64582) 64582..64812 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
N0470_RS22925 (65108) 65108..65398 + 291 WP_000111771.1 hypothetical protein -
N0470_RS22930 (65388) 65388..66287 + 900 WP_000963206.1 nucleotide-binding protein -
N0470_RS22935 (66337) 66337..68562 - 2226 WP_000698737.1 P-loop NTPase fold protein -
N0470_RS22940 (68564) 68564..69652 - 1089 WP_000952217.1 transcriptional repressor PifC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid dfrA12 / aadA2 / qacE / mph(A) / blaTEM-1B / aph(3')-IIa / sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / floR / rmtB / blaCTX-M-55 - 1..162761 162761


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T257613 WP_001044768.1 NZ_CP104327:c64585-64169 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT257613 WP_001261287.1 NZ_CP104327:c64812-64582 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References