Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 2372..3015 Replicon plasmid pKP81324
Accession NZ_CP104316
Organism Klebsiella pneumoniae strain KP8132

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag N2X67_RS28020 Protein ID WP_001044770.1
Coordinates 2372..2788 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag N2X67_RS28025 Protein ID WP_001261282.1
Coordinates 2785..3015 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N2X67_RS28015 (N2X67_28015) 742..2299 - 1558 Protein_1 AAA family ATPase -
N2X67_RS28020 (N2X67_28020) 2372..2788 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
N2X67_RS28025 (N2X67_28025) 2785..3015 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
N2X67_RS28030 (N2X67_28030) 2972..3388 + 417 WP_164481821.1 hypothetical protein -
N2X67_RS28035 (N2X67_28035) 3933..4754 + 822 WP_004213562.1 hypothetical protein -
N2X67_RS28040 (N2X67_28040) 4751..5530 + 780 WP_004213560.1 site-specific integrase -
N2X67_RS28045 (N2X67_28045) 5675..6604 - 930 WP_004213558.1 Rpn family recombination-promoting nuclease/putative transposase -
N2X67_RS28050 (N2X67_28050) 6908..7075 + 168 Protein_8 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iutA / iutA / iucD / iucC / iucB / iucA / iroN 1..194876 194876


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T257578 WP_001044770.1 NZ_CP104316:c2788-2372 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT257578 WP_001261282.1 NZ_CP104316:c3015-2785 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References