Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5325064..5325689 Replicon chromosome
Accession NZ_CP104312
Organism Klebsiella pneumoniae strain KP8132

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag N2X67_RS26215 Protein ID WP_002882817.1
Coordinates 5325064..5325447 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag N2X67_RS26220 Protein ID WP_004150355.1
Coordinates 5325447..5325689 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N2X67_RS26200 (N2X67_26200) 5322430..5323332 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
N2X67_RS26205 (N2X67_26205) 5323329..5323964 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
N2X67_RS26210 (N2X67_26210) 5323961..5324890 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
N2X67_RS26215 (N2X67_26215) 5325064..5325447 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
N2X67_RS26220 (N2X67_26220) 5325447..5325689 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
N2X67_RS26225 (N2X67_26225) 5325894..5326811 + 918 WP_002882812.1 alpha/beta hydrolase -
N2X67_RS26230 (N2X67_26230) 5326825..5327766 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
N2X67_RS26235 (N2X67_26235) 5327811..5328248 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
N2X67_RS26240 (N2X67_26240) 5328245..5329105 - 861 WP_002882807.1 virulence factor BrkB family protein -
N2X67_RS26245 (N2X67_26245) 5329099..5329698 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T257570 WP_002882817.1 NZ_CP104312:c5325447-5325064 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT257570 WP_004150355.1 NZ_CP104312:c5325689-5325447 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References