Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4738254..4739064 Replicon chromosome
Accession NZ_CP104312
Organism Klebsiella pneumoniae strain KP8132

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag N2X67_RS23390 Protein ID WP_002887280.1
Coordinates 4738254..4738787 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag N2X67_RS23395 Protein ID WP_002887278.1
Coordinates 4738798..4739064 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N2X67_RS23385 (N2X67_23385) 4737085..4738206 + 1122 WP_002887282.1 cupin domain-containing protein -
N2X67_RS23390 (N2X67_23390) 4738254..4738787 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
N2X67_RS23395 (N2X67_23395) 4738798..4739064 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
N2X67_RS23400 (N2X67_23400) 4739167..4740600 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
N2X67_RS23405 (N2X67_23405) 4740590..4741273 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
N2X67_RS23410 (N2X67_23410) 4741445..4742830 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
N2X67_RS23415 (N2X67_23415) 4742848..4743192 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T257567 WP_002887280.1 NZ_CP104312:c4738787-4738254 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT257567 WP_002887278.1 NZ_CP104312:c4739064-4738798 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References