Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4943623..4944120 Replicon chromosome
Accession NZ_CP104309
Organism Escherichia coli O157:H7 strain 380-94

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag NUE46_RS24795 Protein ID WP_001173343.1
Coordinates 4943623..4943796 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag NUE46_RS24800 Protein ID WP_000398619.1
Coordinates 4943824..4944120 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE46_RS24785 (4940293) 4940293..4941750 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
NUE46_RS24790 (4941987) 4941987..4943504 + 1518 WP_001295074.1 lysine--tRNA ligase -
NUE46_RS24795 (4943623) 4943623..4943796 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
NUE46_RS24800 (4943824) 4943824..4944120 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
NUE46_RS24805 (4944347) 4944347..4944619 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
NUE46_RS24810 (4944631) 4944631..4944861 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
NUE46_RS24815 (4945042) 4945042..4946673 + 1632 WP_001216477.1 sensor histidine kinase -
NUE46_RS24820 (4946670) 4946670..4947389 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T257553 WP_001173343.1 NZ_CP104309:c4943796-4943623 [Escherichia coli O157:H7]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT257553 WP_000398619.1 NZ_CP104309:c4944120-4943824 [Escherichia coli O157:H7]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References