Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4105909..4106527 Replicon chromosome
Accession NZ_CP104309
Organism Escherichia coli O157:H7 strain 380-94

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag NUE46_RS20940 Protein ID WP_001291435.1
Coordinates 4106309..4106527 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag NUE46_RS20935 Protein ID WP_000344800.1
Coordinates 4105909..4106283 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE46_RS20925 (4100998) 4100998..4102191 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
NUE46_RS20930 (4102214) 4102214..4105363 + 3150 WP_001132452.1 efflux RND transporter permease AcrB -
NUE46_RS20935 (4105909) 4105909..4106283 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
NUE46_RS20940 (4106309) 4106309..4106527 + 219 WP_001291435.1 HHA domain-containing protein Toxin
NUE46_RS20945 (4106699) 4106699..4107250 + 552 WP_000102553.1 maltose O-acetyltransferase -
NUE46_RS20950 (4107366) 4107366..4107836 + 471 WP_000136192.1 YlaC family protein -
NUE46_RS20955 (4108000) 4108000..4109550 + 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
NUE46_RS20960 (4109592) 4109592..4109945 - 354 WP_000878140.1 DUF1428 domain-containing protein -
NUE46_RS20970 (4110324) 4110324..4110635 + 312 WP_000409911.1 MGMT family protein -
NUE46_RS20975 (4110668) 4110668..4111240 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T257547 WP_001291435.1 NZ_CP104309:4106309-4106527 [Escherichia coli O157:H7]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT257547 WP_000344800.1 NZ_CP104309:4105909-4106283 [Escherichia coli O157:H7]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References