Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 890207..890861 Replicon chromosome
Accession NZ_CP104309
Organism Escherichia coli O157:H7 strain 380-94

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag NUE46_RS04440 Protein ID WP_000244781.1
Coordinates 890454..890861 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag NUE46_RS04435 Protein ID WP_000354046.1
Coordinates 890207..890473 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE46_RS04415 (886472) 886472..887728 - 1257 WP_009671380.1 family 1 glycosylhydrolase -
NUE46_RS04420 (887773) 887773..888084 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
NUE46_RS04425 (888248) 888248..888907 + 660 WP_000250274.1 hemolysin III family protein -
NUE46_RS04430 (888984) 888984..889964 - 981 WP_000886053.1 tRNA-modifying protein YgfZ -
NUE46_RS04435 (890207) 890207..890473 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
NUE46_RS04440 (890454) 890454..890861 + 408 WP_000244781.1 protein YgfX Toxin
NUE46_RS04445 (890901) 890901..891422 - 522 WP_001055874.1 flavodoxin FldB -
NUE46_RS04450 (891534) 891534..892430 + 897 WP_000806640.1 site-specific tyrosine recombinase XerD -
NUE46_RS04455 (892455) 892455..893165 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
NUE46_RS04460 (893171) 893171..894904 + 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T257533 WP_000244781.1 NZ_CP104309:890454-890861 [Escherichia coli O157:H7]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT257533 WP_000354046.1 NZ_CP104309:890207-890473 [Escherichia coli O157:H7]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References