Detailed information of TA system TA025753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2102142..2102718
• Replicon: chromosome
• Accession: NC_016612
• Organism: Klebsiella oxytoca KCTC 1686

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: KOX_RS09865
• Protein ID: WP_071881753.1
• Coordinates: 2102142..2102429 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: KOX_RS09870
• Protein ID: WP_014227757.1
• Coordinates: 2102416..2102718 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOX_RS09835	2097483..2098001	-	519	WP_004098240.1	hypothetical protein	-
KOX_RS09840	2097998..2098798	-	801	WP_014227752.1	winged helix-turn-helix domain-containing protein	-
KOX_RS09845	2098944..2099528	-	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
KOX_RS09850	2099676..2100458	-	783	WP_014227753.1	NAD(P)H-dependent oxidoreductase	-
KOX_RS09855	2100546..2101454	+	909	WP_014227754.1	LysR family transcriptional regulator	-
KOX_RS09860	2101448..2101891	+	444	WP_014227755.1	FosA family fosfomycin resistance glutathione transferase	-
KOX_RS09865	2102142..2102429	+	288	WP_071881753.1	BrnT family toxin	Toxin
KOX_RS09870	2102416..2102718	+	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
KOX_RS09875	2102840..2104183	-	1344	WP_014227758.1	APC family permease	-
KOX_RS09880	2104180..2105091	-	912	WP_014227759.1	acetamidase/formamidase family protein	-
KOX_RS09885	2105545..2105880	-	336	WP_014227760.1	endoribonuclease SymE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11239.69 Da
• Isoelectric Point: 8.5899

>T25753 WP_071881753.1 NC_016612:2102142-2102429 [Klebsiella michiganensis KCTC 1686]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T25753 NC_016612:2102142-2102429 [Klebsiella michiganensis KCTC 1686]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGGTCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT25753 WP_014227757.1 NC_016612:2102416-2102718 [Klebsiella michiganensis KCTC 1686]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT25753 NC_016612:2102416-2102718 [Klebsiella michiganensis KCTC 1686]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591