Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 351953..352753 Replicon chromosome
Accession NZ_CP104309
Organism Escherichia coli O157:H7 strain 380-94

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag NUE46_RS01705 Protein ID WP_000342448.1
Coordinates 352226..352753 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag NUE46_RS01700 Protein ID WP_001277108.1
Coordinates 351953..352219 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE46_RS01680 (347611) 347611..348279 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
NUE46_RS01685 (348272) 348272..349330 + 1059 WP_001042018.1 permease-like cell division protein FtsX -
NUE46_RS01690 (349575) 349575..350429 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
NUE46_RS01695 (350700) 350700..351803 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
NUE46_RS01700 (351953) 351953..352219 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
NUE46_RS01705 (352226) 352226..352753 + 528 WP_000342448.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
NUE46_RS01710 (352750) 352750..353133 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
NUE46_RS01715 (353557) 353557..354666 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
NUE46_RS01720 (354714) 354714..355640 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
NUE46_RS01725 (355637) 355637..356914 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
NUE46_RS01730 (356911) 356911..357678 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T257529 WP_000342448.1 NZ_CP104309:352226-352753 [Escherichia coli O157:H7]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT257529 WP_001277108.1 NZ_CP104309:351953-352219 [Escherichia coli O157:H7]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References