Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 309522..310153 Replicon chromosome
Accession NZ_CP104309
Organism Escherichia coli O157:H7 strain 380-94

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag NUE46_RS01455 Protein ID WP_001259386.1
Coordinates 309522..309797 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag NUE46_RS01460 Protein ID WP_000593555.1
Coordinates 309794..310153 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NUE46_RS01440 (304526) 304526..305593 + 1068 WP_000361458.1 HlyD family secretion protein -
NUE46_RS01445 (305590) 305590..308325 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
NUE46_RS01450 (308325) 308325..309449 + 1125 WP_001301659.1 ABC transporter permease -
NUE46_RS01455 (309522) 309522..309797 + 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
NUE46_RS01460 (309794) 309794..310153 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
NUE46_RS01465 (310203) 310203..311063 - 861 WP_001131788.1 class II aldolase -
NUE46_RS01470 (311095) 311095..311364 - 270 WP_000933060.1 HPr family phosphocarrier protein -
NUE46_RS01475 (311354) 311354..312862 - 1509 WP_001301773.1 FGGY-family carbohydrate kinase -
NUE46_RS01480 (312855) 312855..314213 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
NUE46_RS01485 (314290) 314290..314571 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
NUE46_RS01490 (314568) 314568..315041 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 300666..310153 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T257528 WP_001259386.1 NZ_CP104309:309522-309797 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT257528 WP_000593555.1 NZ_CP104309:309794-310153 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References