Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2768316..2768967 Replicon chromosome
Accession NZ_CP104303
Organism Lacticaseibacillus paracasei strain VHProbi O44

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag N4T21_RS13605 Protein ID WP_003567661.1
Coordinates 2768316..2768699 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag N4T21_RS13610 Protein ID WP_003567665.1
Coordinates 2768719..2768967 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N4T21_RS13600 (N4T21_13600) 2767611..2768132 - 522 WP_003567657.1 QueT transporter family protein -
N4T21_RS13605 (N4T21_13605) 2768316..2768699 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
N4T21_RS13610 (N4T21_13610) 2768719..2768967 - 249 WP_003567665.1 antitoxin Antitoxin
N4T21_RS13615 (N4T21_13615) 2769035..2770171 - 1137 WP_003581082.1 alanine racemase -
N4T21_RS13620 (N4T21_13620) 2770158..2770532 - 375 WP_003567669.1 holo-ACP synthase -
N4T21_RS13625 (N4T21_13625) 2770701..2772209 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
N4T21_RS13630 (N4T21_13630) 2772335..2772478 - 144 WP_016380391.1 hypothetical protein -
N4T21_RS13635 (N4T21_13635) 2772509..2773897 - 1389 WP_003581096.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T257526 WP_003567661.1 NZ_CP104303:c2768699-2768316 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT257526 WP_003567665.1 NZ_CP104303:c2768967-2768719 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References