Detailed information of TA system TA025752

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 2045451..2046141
• Replicon: chromosome
• Accession: NC_016612
• Organism: Klebsiella oxytoca KCTC 1686

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: KOX_RS09605
• Protein ID: WP_041165267.1
• Coordinates: 2045800..2046141 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A0H3H5M8
• Locus tag: KOX_RS28040
• Protein ID: WP_014227717.1
• Coordinates: 2045451..2045777 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOX_RS09575	2040967..2042055	+	1089	WP_014227712.1	hypothetical protein	-
KOX_RS09580	2042137..2043027	+	891	WP_014227713.1	50S ribosome-binding GTPase	-
KOX_RS09585	2043591..2044280	+	690	WP_041165265.1	hypothetical protein	-
KOX_RS09590	2044504..2044950	+	447	WP_014227715.1	antirestriction protein	-
KOX_RS09595	2044961..2045437	+	477	WP_014227716.1	DNA repair protein RadC	-
KOX_RS28040	2045451..2045777	+	327	WP_014227717.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KOX_RS09605	2045800..2046141	+	342	WP_041165267.1	TA system toxin CbtA family protein	Toxin
KOX_RS09610	2046256..2047089	+	834	WP_014227719.1	DUF4942 domain-containing protein	-
KOX_RS29825	2047293..2048288	+	996	WP_014227720.1	hypothetical protein	-
KOX_RS09620	2048598..2049341	-	744	WP_014227721.1	hypothetical protein	-
KOX_RS09625	2049507..2049941	-	435	WP_004098173.1	VOC family protein	-
KOX_RS09630	2050088..2050411	-	324	WP_014227722.1	endoribonuclease SymE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2020549..2050411	29862

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12768.64 Da
• Isoelectric Point: 7.9093

>T25752 WP_041165267.1 NC_016612:2045800-2046141 [Klebsiella michiganensis KCTC 1686]
MKTLPATTQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLADAVNFLVEKYELIRIDRRGF
NCQEQSPYLGAADILRARQATGLLRQSHHPSIR

Nucleotide

• Length: 342 bp

>T25752 NC_016612:2045800-2046141 [Klebsiella michiganensis KCTC 1686]
ATGAAAACCTTACCTGCAACAACTCAGCGGGCGGCTAAGCCGTGCCCGTCTCCCGTGACGGTCTGGCAGATGCTACTTAC
CCGTCTGCTGGAGCAACATTATGGCCTTACCCTGAATGACACGCCGTTCAGCGATGAAACCGTTATTCAGGAACATATCA
ACGCCGGTATTACGCTGGCTGATGCTGTTAACTTTCTGGTGGAAAAGTACGAACTGATTCGTATCGATCGCAGAGGGTTT
AACTGTCAGGAGCAGTCCCCTTATCTGGGCGCAGCGGATATTTTGCGGGCGCGGCAGGCAACTGGCCTGTTGCGGCAAAG
CCATCATCCTTCGATACGCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12298.02 Da
• Isoelectric Point: 6.9563

>AT25752 WP_014227717.1 NC_016612:2045451-2045777 [Klebsiella michiganensis KCTC 1686]
MPLTDGIQWGLKRSFTPNFGARLVQEGNRLHNLADRANITGKFVDVECQKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT25752 NC_016612:2045451-2045777 [Klebsiella michiganensis KCTC 1686]
ATGCCACTGACTGATGGCATACAGTGGGGATTAAAGCGAAGTTTCACACCGAATTTTGGCGCAAGGCTGGTACAGGAAGG
CAACCGACTGCACAACCTGGCTGACCGGGCCAATATCACCGGTAAATTTGTTGATGTCGAATGTCAGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGTAGTTGTGGTTATGTCTACATCGTTATTTATCCCACTCA
ACGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H5M8