25750

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1314463..1315010 Replicon chromosome
Accession NC_016612
Organism Klebsiella oxytoca KCTC 1686

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag KOX_RS06220 Protein ID WP_014227299.1
Coordinates 1314702..1315010 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag KOX_RS06215 Protein ID WP_014227298.1
Coordinates 1314463..1314699 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KOX_RS06200 1310670..1311836 + 1167 WP_014227296.1 multidrug effflux MFS transporter -
KOX_RS06205 1311997..1312899 + 903 WP_009654080.1 N-acetylmuramic acid 6-phosphate etherase -
KOX_RS06210 1312924..1314357 + 1434 WP_014227297.1 PTS N-acetylmuramic acid transporter subunit IIBC -
KOX_RS06215 1314463..1314699 + 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KOX_RS06220 1314702..1315010 + 309 WP_014227299.1 CcdB family protein Toxin
KOX_RS06225 1315161..1316063 + 903 WP_014227300.1 EamA family transporter -
KOX_RS06230 1316067..1316324 - 258 WP_038422923.1 hypothetical protein -
KOX_RS06235 1316324..1317313 - 990 WP_014227301.1 Rpn family recombination-promoting nuclease/putative transposase -
KOX_RS06240 1317523..1317816 + 294 WP_014227302.1 hypothetical protein -
KOX_RS06245 1317840..1318781 - 942 WP_014227303.1 LacI family transcriptional regulator -
KOX_RS06250 1319076..1319387 + 312 WP_014227304.1 PTS sugar transporter subunit IIB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T25750 WP_014227299.1 NC_016612:1314702-1315010 [Klebsiella michiganensis KCTC 1686]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T25750 NC_016612:1314702-1315010 [Klebsiella michiganensis KCTC 1686]
ATGCAGTATTACGTTTATAAGAATACAGGTCGAATTGCTGCGTATCCTTACCTCCTTGATGTACAGAGTGACATTATCGG
CAAGCGCAATACTCGGGTCGTGATTCCACTGTTTCCACTGAAAAACTATAAAGGTCCACGCGCCGATCGTTTAACACCGC
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACATCGTGTTCTGGGTGAAGAA
GTTTGTAATCTGAATCACCAGCGCGAAGTCGTTAAAGCTTCAATGGATTTTCTTTTCGACGGGATCTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT25750 WP_014227298.1 NC_016612:1314463-1314699 [Klebsiella michiganensis KCTC 1686]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT25750 NC_016612:1314463-1314699 [Klebsiella michiganensis KCTC 1686]
ATGCCAGCCGCAAAACGCAATAAGCAGAACGTCAGCGTGACACTGGACCCAGGCCTGGTCAAACAGGCTCGTGAGGTCGG
CATCAATCTGTCTCTAACGTTGACTAACGCTCTAAAAAGCGAAATCCACAGCCTCGAAATAGCCCGCTGGCAAAAAGAAA
ATGCCCAGGCAATCGACTACCTCAATAATTTAACTGAGGAAAATGGACTTCTCTCTGATGAATACAGGACGTTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References