Detailed information of TA system TA025748

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 454889..455589
• Replicon: chromosome
• Accession: NC_016612
• Organism: Klebsiella oxytoca KCTC 1686

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A0H3GY64
• Locus tag: KOX_RS02135
• Protein ID: WP_014226745.1
• Coordinates: 454889..455212 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KOX_RS27660
• Protein ID: WP_061157164.1
• Coordinates: 455233..455589 (-)
• Length: 119 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KOX_RS02115	450301..451521	+	1221	WP_014226740.1	MFS transporter	-
KOX_RS02120	451508..451921	+	414	WP_014226741.1	carboxymuconolactone decarboxylase family protein	-
KOX_RS02125	451918..453093	+	1176	WP_014226742.1	acetyl-CoA C-acetyltransferase	-
KOX_RS02130	453183..454181	-	999	WP_038422989.1	aldo/keto reductase	-
KOX_RS02135	454889..455212	-	324	WP_014226745.1	TA system toxin CbtA family protein	Toxin
KOX_RS27660	455233..455589	-	357	WP_061157164.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KOX_RS02145	455615..455836	-	222	WP_014226747.1	DUF987 domain-containing protein	-
KOX_RS02150	455833..456375	-	543	WP_032949998.1	DNA repair protein RadC	-
KOX_RS02155	456388..456831	-	444	WP_014226749.1	antirestriction protein	-
KOX_RS02160	456862..457683	-	822	WP_014226750.1	DUF945 domain-containing protein	-
KOX_RS27665	457782..458012	-	231	WP_014226751.1	DUF905 domain-containing protein	-
KOX_RS02165	458084..458533	-	450	WP_014226752.1	hypothetical protein	-
KOX_RS02170	458530..458982	-	453	WP_000737933.1	hypothetical protein	-
KOX_RS02175	459019..459588	-	570	WP_014226754.1	hypothetical protein	-
KOX_RS02180	459588..460292	-	705	WP_014226755.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	447789..477388	29599

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12466.52 Da
• Isoelectric Point: 7.1331

>T25748 WP_014226745.1 NC_016612:c455212-454889 [Klebsiella michiganensis KCTC 1686]
MKFLPATNMRAAKPCLSPVTIWQMLLSRLLEQHYGLTLNDTPFCDETVIQEHIDAGITLANAINFLVEKYELVRIDRRGF
SWQEQTPYLTIIDIMRARRDLGLMNRN

Nucleotide

• Length: 324 bp

>T25748 NC_016612:c455212-454889 [Klebsiella michiganensis KCTC 1686]
ATGAAATTTCTACCTGCAACAAATATGCGGGCGGCGAAGCCATGCCTGTCGCCCGTGACTATCTGGCAAATGCTACTTAG
TCGTCTGCTGGAACAGCATTATGGCCTGACACTAAACGATACACCTTTCTGTGATGAAACCGTCATACAGGAACATATCG
ATGCCGGTATCACTCTCGCTAATGCCATTAACTTTCTAGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTT
AGCTGGCAAGAACAAACGCCATATCTCACGATTATTGATATCATGAGGGCCCGTCGCGATCTGGGTTTAATGAATCGTAA
TTAG

Antitoxin

Protein

• Length: 119 a.a.
• Molecular weight: 13163.93 Da
• Isoelectric Point: 6.6215

>AT25748 WP_061157164.1 NC_016612:c455589-455233 [Klebsiella michiganensis KCTC 1686]
MNNRSESGAIPENPACQQWGLKRAMTPCFGARLVQEGDRLHFLADRAGFNGAFSDEHALRLDQAFPLILKQLELILTSGE
LSPRHQHCVTLYHNGLTCEADTLGSCGYVYITIYPTQR

Nucleotide

• Length: 357 bp

>AT25748 NC_016612:c455589-455233 [Klebsiella michiganensis KCTC 1686]
ATGAACAATCGCTCAGAATCCGGTGCCATACCGGAGAATCCCGCCTGCCAGCAATGGGGGCTGAAACGTGCTATGACCCC
CTGTTTTGGAGCACGTCTGGTGCAGGAAGGCGACCGCCTGCATTTTCTCGCGGACCGCGCCGGGTTTAACGGTGCTTTCA
GCGACGAGCATGCGTTGCGCCTCGACCAGGCCTTTCCGTTGATACTCAAGCAGCTGGAGCTGATACTCACCAGCGGAGAA
CTCTCCCCCCGCCATCAGCACTGTGTCACCCTCTATCACAACGGACTCACCTGTGAAGCCGATACGCTTGGTAGTTGTGG
CTACGTATACATCACCATTTATCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GY64