Detailed information of TA system TA025747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 892026..892690
• Replicon: chromosome
• Accession: NC_016604
• Organism: Mycobacterium rhodesiae NBB3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MYCRHN_RS04265
• Protein ID: WP_014209314.1
• Coordinates: 892026..892436 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G8RRQ9
• Locus tag: MYCRHN_RS04270
• Protein ID: WP_014209315.1
• Coordinates: 892433..892690 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MYCRHN_RS04250	887775..888407	-	633	WP_014209311.1	hypothetical protein	-
MYCRHN_RS04255	888404..890809	-	2406	WP_014209312.1	cbb3-type cytochrome c oxidase subunit I	-
MYCRHN_RS04260	891235..891921	+	687	WP_014209313.1	sensor domain-containing protein	-
MYCRHN_RS04265	892026..892436	-	411	WP_014209314.1	type II toxin-antitoxin system VapC family toxin	Toxin
MYCRHN_RS04270	892433..892690	-	258	WP_014209315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MYCRHN_RS04275	892956..893339	-	384	WP_041301348.1	hypothetical protein	-
MYCRHN_RS04280	893663..894535	-	873	WP_041301350.1	hypothetical protein	-
MYCRHN_RS32010	894730..894885	-	156	WP_014209318.1	hypothetical protein	-
MYCRHN_RS04285	894985..895872	-	888	WP_014209319.1	hypothetical protein	-
MYCRHN_RS04290	895938..896606	-	669	WP_014209320.1	NUDIX hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (10-126)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14433.49 Da
• Isoelectric Point: 4.2778

>T25747 WP_014209314.1 NC_016604:c892436-892026 [Mycolicibacterium rhodesiae NBB3]
VTTEPPTAGILDTSVFIASETGRQLDTTLIPDEVATTVVTLAELNAGVLAAKTSEIRARRLRTLDAVADMTALPVDEDAA
QLWALLRVHLAEAGRRVRVNDLWIAAIAAAKQLPVVTQDEDFDALEGVAGLAIVHV

Nucleotide

• Length: 411 bp

>T25747 NC_016604:c892436-892026 [Mycolicibacterium rhodesiae NBB3]
GTGACGACCGAGCCCCCGACGGCCGGCATCCTCGACACGTCGGTCTTCATCGCCAGCGAAACCGGGCGCCAACTAGACAC
GACGTTGATTCCCGACGAAGTAGCCACCACTGTGGTGACCCTCGCCGAACTGAACGCCGGTGTCCTCGCGGCGAAAACCT
CGGAGATCCGCGCGCGTCGACTTCGCACGTTGGATGCCGTTGCTGACATGACCGCGCTGCCGGTCGACGAAGATGCGGCT
CAACTGTGGGCGCTGCTCCGCGTTCACCTGGCCGAGGCGGGCCGACGGGTCCGAGTCAATGACCTATGGATTGCAGCGAT
CGCAGCCGCCAAGCAGCTGCCGGTCGTCACGCAGGATGAGGACTTCGATGCCCTAGAGGGTGTCGCGGGTCTCGCAATTG
TCCACGTCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9332.52 Da
• Isoelectric Point: 4.6702

>AT25747 WP_014209315.1 NC_016604:c892690-892433 [Mycolicibacterium rhodesiae NBB3]
MSTVASRELRNDTAGVLRRVEAGEEVTITVNGRAVALITAVTPKRRRWLSKAELIKRLETSQADPGLRDDLAELAGETTD
ELDDL

Nucleotide

• Length: 258 bp

>AT25747 NC_016604:c892690-892433 [Mycolicibacterium rhodesiae NBB3]
ATGTCTACGGTCGCATCCCGCGAGCTGCGAAACGACACCGCGGGCGTGCTTCGTCGGGTCGAGGCAGGCGAGGAGGTGAC
GATCACGGTCAACGGGAGAGCCGTCGCGCTGATAACTGCTGTGACGCCGAAGCGTCGGAGGTGGCTGTCGAAGGCGGAGT
TGATCAAGCGCCTCGAGACCAGTCAGGCGGACCCCGGTTTGCGTGACGACCTCGCGGAGCTCGCCGGCGAAACCACCGAC
GAACTCGACGACTTGTGA

Structures

Antitoxin

• AlphaFold DB: G8RRQ9