Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1660767..1661564 Replicon chromosome
Accession NZ_CP104284
Organism Enterobacter hormaechei subsp. steigerwaltii strain JT.WLB1A

Toxin (Protein)


Gene name KacT Uniprot ID A0A4Q2R3N2
Locus tag N2K85_RS07755 Protein ID WP_032670168.1
Coordinates 1661043..1661564 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A331QJY7
Locus tag N2K85_RS07750 Protein ID WP_015570876.1
Coordinates 1660767..1661036 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N2K85_RS07715 (N2K85_07715) 1656308..1656667 + 360 WP_017384779.1 helix-turn-helix domain-containing protein -
N2K85_RS07720 (N2K85_07720) 1656739..1656840 - 102 Protein_1495 transcriptional regulator -
N2K85_RS07725 (N2K85_07725) 1656993..1658234 + 1242 WP_017693667.1 bifunctional glucose-1-phosphatase/inositol phosphatase -
N2K85_RS07730 (N2K85_07730) 1658267..1658494 - 228 WP_006809324.1 YccJ family protein -
N2K85_RS07735 (N2K85_07735) 1658513..1659109 - 597 WP_006809323.1 NAD(P)H:quinone oxidoreductase -
N2K85_RS07740 (N2K85_07740) 1659501..1659671 + 171 WP_001273664.1 general stress protein -
N2K85_RS07745 (N2K85_07745) 1659788..1660693 + 906 WP_017693666.1 DMT family transporter -
N2K85_RS07750 (N2K85_07750) 1660767..1661036 + 270 WP_015570876.1 DUF1778 domain-containing protein Antitoxin
N2K85_RS07755 (N2K85_07755) 1661043..1661564 + 522 WP_032670168.1 GNAT family N-acetyltransferase Toxin
N2K85_RS07760 (N2K85_07760) 1661623..1662945 - 1323 WP_260673320.1 pyrimidine utilization transport protein G -
N2K85_RS07765 (N2K85_07765) 1662967..1663458 - 492 WP_040117474.1 pyrimidine utilization flavin reductase protein F -
N2K85_RS07770 (N2K85_07770) 1663471..1664061 - 591 WP_040117475.1 malonic semialdehyde reductase -
N2K85_RS07775 (N2K85_07775) 1664071..1664871 - 801 WP_047726857.1 pyrimidine utilization protein D -
N2K85_RS07780 (N2K85_07780) 1664879..1665265 - 387 WP_040117477.1 pyrimidine utilization protein C -
N2K85_RS07785 (N2K85_07785) 1665277..1665966 - 690 WP_023296288.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19437.18 Da        Isoelectric Point: 7.4318

>T257462 WP_032670168.1 NZ_CP104284:1661043-1661564 [Enterobacter hormaechei subsp. steigerwaltii]
VANLTIEMLSEGTDYDFGDFDCGEPSLNAFLTEHLVRQHGGRILRGYLLKERDHPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAVHKELQGNEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKLGFIPLAGENSSSLF
FPTQSIERLFEQA

Download         Length: 522 bp

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10247.75 Da        Isoelectric Point: 5.8836

>AT257462 WP_015570876.1 NZ_CP104284:1660767-1661036 [Enterobacter hormaechei subsp. steigerwaltii]
MPALKKQRIDLRLSDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWIAVMDAIDNPPEPNERLKR
AAKRLRNME

Download         Length: 270 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q2R3N2

Antitoxin

Source ID Structure
AlphaFold DB A0A331QJY7

References