257427

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 1896665..1898173 Replicon chromosome
Accession NZ_CP104254
Organism Pseudomonas aeruginosa strain PALA1

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag PALA1_RS08950 Protein ID WP_003119682.1
Coordinates 1896665..1897252 (+) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A1C7C4U7
Locus tag PALA1_RS08955 Protein ID WP_003091607.1
Coordinates 1897319..1898173 (+) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PALA1_RS08945 (PALA1_01773) 1892920..1896399 - 3480 WP_003091610.1 PAS domain-containing hybrid sensor histidine kinase/response regulator -
PALA1_RS08950 (PALA1_01774) 1896665..1897252 + 588 WP_003119682.1 GNAT family protein Toxin
PALA1_RS08955 (PALA1_01775) 1897319..1898173 + 855 WP_003091607.1 AraC family transcriptional regulator Antitoxin
PALA1_RS08960 (PALA1_01776) 1898270..1900435 + 2166 WP_021265073.1 TonB-dependent receptor family protein -
PALA1_RS08965 (PALA1_01777) 1900610..1902484 + 1875 WP_003091605.1 MFS transporter -
PALA1_RS08970 1902545..1902754 - 210 Protein_1779 cold-shock protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T257427 WP_003119682.1 NZ_CP104254:1896665-1897252 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31895.20 Da        Isoelectric Point: 7.5295

>AT257427 WP_003091607.1 NZ_CP104254:1897319-1898173 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETMLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Download         Length: 855 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB A0A1C7C4U7

References