Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1765780..1766378 Replicon chromosome
Accession NC_016593
Organism Geobacillus thermoleovorans CCB US3 UF5

Toxin (Protein)


Gene name mazF Uniprot ID G8N5V4
Locus tag GTCCBUS3UF5_RS08725 Protein ID WP_011231143.1
Coordinates 1766049..1766378 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G8N5V3
Locus tag GTCCBUS3UF5_RS08720 Protein ID WP_011231142.1
Coordinates 1765780..1766049 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTCCBUS3UF5_RS08685 1761480..1762460 + 981 WP_025039054.1 ABC transporter permease -
GTCCBUS3UF5_RS08690 1762529..1762996 - 468 WP_021321939.1 hypothetical protein -
GTCCBUS3UF5_RS08695 1763010..1763774 - 765 WP_011231140.1 hypothetical protein -
GTCCBUS3UF5_RS08700 1763901..1764956 + 1056 WP_011231141.1 transposase -
GTCCBUS3UF5_RS08720 1765780..1766049 + 270 WP_011231142.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GTCCBUS3UF5_RS08725 1766049..1766378 + 330 WP_011231143.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GTCCBUS3UF5_RS08730 1766831..1767664 + 834 WP_011231146.1 pirin family protein -
GTCCBUS3UF5_RS08735 1767760..1768509 + 750 WP_011231147.1 oxygen-insensitive NADPH nitroreductase -
GTCCBUS3UF5_RS08740 1768699..1769442 + 744 WP_011231148.1 NAD-dependent protein deacylase -
GTCCBUS3UF5_RS08745 1769622..1770800 + 1179 WP_011231149.1 acyl-CoA dehydrogenase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-107)

Antitoxin

Antitoxin PemI-like

(12-87)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12071.86 Da        Isoelectric Point: 7.1331

>T25741 WP_011231143.1 NC_016593:1766049-1766378 [Geobacillus thermoleovorans CCB_US3_UF5]
MQAPDRGDLVYVNFNPQAGHEQAGKRPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVELPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHTFLS

Download         Length: 330 bp

>T25741 NC_016593:1766049-1766378 [Geobacillus thermoleovorans CCB_US3_UF5]
ATGCAAGCGCCGGATCGTGGGGATCTTGTTTACGTCAATTTCAATCCACAAGCAGGGCATGAGCAGGCAGGAAAAAGGCC
CGGCATCGTTCTATCACCAAAGCGGTTCAATCAATTGACAGGGTTTGCGGTGCTTTGCCCGATCACCCGGCAACAAAAAG
GATATCCATTTGAAGTGGAATTGCCATCAGGCTTGGCCGTTGAAGGCGTGATTTTAACCGATCAGGTCAAAAGTTTAGAT
TGGCGCGCCCGACAGCTTCAAATAGTAGGACGGGCGCCCAATGAAGTTGTTTCGGATTGCTTGGATCTGATTCATACTTT
TCTTTCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9992.67 Da        Isoelectric Point: 7.2050

>AT25741 WP_011231142.1 NC_016593:1765780-1766049 [Geobacillus thermoleovorans CCB_US3_UF5]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEMIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Download         Length: 270 bp

>AT25741 NC_016593:1765780-1766049 [Geobacillus thermoleovorans CCB_US3_UF5]
ATGGAATTAAACAAACAGAAAGGAGTGTCAATCATGACAATAACAGTTCAAAAATGGGGAAACAGCCTTGCGGTTCGTAT
CCCAAGCGTGATTGCTGAACGTTTAGCGCTTCATCAAGGATCAGAAGTGGAGATGATCGTTGAGAACCAAGCGATCAAGT
TGATCCCGAAAAAGAAAAAGCCGACATTGGAGGAACTTTTGGCTAAAATCACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9EUB7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9HWH2

References