Detailed information of TA system TA025740

Bioinformatically predicted

Overview

TA module

• Type: VII
• Classification (family/domain): HepT-MntA/HepT(toxin)
• Location: 1069835..1070651
• Replicon: chromosome
• Accession: NC_016593
• Organism: Geobacillus thermoleovorans CCB US3 UF5

Toxin (Protein)

• Gene name: hepT
• Uniprot ID: G8MYR9
• Locus tag: GTCCBUS3UF5_RS05375
• Protein ID: WP_014195290.1
• Coordinates: 1069835..1070254 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: mntA
• Uniprot ID: G8MYS0
• Locus tag: GTCCBUS3UF5_RS05380
• Protein ID: WP_014195291.1
• Coordinates: 1070244..1070651 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GTCCBUS3UF5_RS05350	1066610..1067125	+	516	WP_014195285.1	GNAT family N-acetyltransferase	-
GTCCBUS3UF5_RS05355	1067197..1068468	+	1272	WP_122983902.1	DUF3533 domain-containing protein	-
GTCCBUS3UF5_RS05360	1068493..1068705	+	213	WP_014195287.1	ATP-binding cassette domain-containing protein	-
GTCCBUS3UF5_RS05365	1068672..1069142	+	471	Protein_1020	ABC transporter permease	-
GTCCBUS3UF5_RS05370	1069154..1069807	+	654	WP_014195289.1	TetR/AcrR family transcriptional regulator	-
GTCCBUS3UF5_RS05375	1069835..1070254	-	420	WP_014195290.1	DUF86 domain-containing protein	Toxin
GTCCBUS3UF5_RS05380	1070244..1070651	-	408	WP_014195291.1	nucleotidyltransferase domain-containing protein	Antitoxin
GTCCBUS3UF5_RS05385	1070932..1073064	-	2133	WP_014195292.1	ATP-dependent Clp protease ATP-binding subunit	-
GTCCBUS3UF5_RS05390	1073332..1074078	+	747	WP_025039183.1	FixH family protein	-
GTCCBUS3UF5_RS05395	1074186..1075226	+	1041	WP_014195294.1	membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease HepT-like (14-131)

Antitoxin

• Polymerase beta, nucleotidyltransferase (9-98)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15907.43 Da
• Isoelectric Point: 8.2859

>T25740 WP_014195290.1 NC_016593:c1070254-1069835 [Geobacillus thermoleovorans CCB_US3_UF5]
MKSDVILNKISVIERCLKRIREEYNGDPKNLQNYTKQDSIVLNLQRACEACIDLAMHIVAEQKFGLPQHSRDAFALLEEH
GVISPSISKKMKAMVGFRNIAVHDYQQLNLGILQAIVEHHLDDFKQFTKAILDYAKKNS

Nucleotide

• Length: 420 bp

>T25740 NC_016593:c1070254-1069835 [Geobacillus thermoleovorans CCB_US3_UF5]
ATGAAAAGTGATGTCATTTTAAACAAGATCAGCGTGATCGAACGCTGCCTGAAACGAATTCGTGAAGAATATAACGGCGA
TCCAAAAAATTTACAAAATTACACAAAACAAGATTCGATCGTTCTGAATTTACAGCGGGCGTGCGAGGCATGCATCGATT
TGGCCATGCATATTGTGGCCGAGCAGAAATTCGGATTGCCGCAGCATAGCCGCGATGCATTCGCTCTCCTCGAAGAACAT
GGGGTCATCTCCCCTTCCATAAGCAAAAAGATGAAGGCGATGGTCGGATTCCGCAACATCGCCGTTCACGACTATCAACA
ACTGAACCTTGGCATCTTGCAAGCCATCGTCGAACACCATCTTGATGATTTCAAACAATTTACGAAAGCCATCCTCGATT
ATGCTAAGAAAAACAGCTAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 15273.46 Da
• Isoelectric Point: 4.8382

>AT25740 WP_014195291.1 NC_016593:c1070651-1070244 [Geobacillus thermoleovorans CCB_US3_UF5]
MPNEMETIIIQTLRPALHPFVIYLFGSAARGTLRPDSDVDIAFVSDGEPHDPYELFRLAGELADKLGRDVDLVDLRQAST
VFQAQVVSTGKAIDCRDERKRAEFEMKTLKMYVKLNEERAPVLKQITESGSIYEK

Nucleotide

• Length: 408 bp

>AT25740 NC_016593:c1070651-1070244 [Geobacillus thermoleovorans CCB_US3_UF5]
TTGCCAAACGAAATGGAGACGATCATCATTCAGACGCTCCGCCCGGCTCTTCACCCGTTCGTCATCTACCTGTTCGGTTC
AGCCGCCCGTGGGACGCTGCGCCCAGACAGCGATGTCGACATCGCCTTTGTCAGCGACGGCGAACCGCATGATCCGTATG
AGCTGTTTCGGCTCGCCGGGGAGTTGGCTGACAAGTTAGGGCGAGATGTCGATCTTGTCGATTTGCGCCAAGCCAGCACC
GTGTTTCAAGCGCAAGTCGTCTCGACGGGAAAAGCCATTGATTGCCGCGACGAGCGGAAACGGGCTGAGTTCGAAATGAA
AACATTGAAAATGTATGTGAAACTAAACGAAGAGAGAGCACCGGTGTTAAAACAAATTACGGAAAGCGGGAGCATATATG
AAAAGTGA

Structures

Toxin

• AlphaFold DB: G8MYR9

Antitoxin

• AlphaFold DB: A0A7U9EPL2