257396

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1183861..1184698 Replicon chromosome
Accession NZ_CP104206
Organism Escherichia coli strain hubt-sl

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag N1H45_RS05725 Protein ID WP_000227784.1
Coordinates 1183861..1184403 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag N1H45_RS05730 Protein ID WP_001297137.1
Coordinates 1184387..1184698 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1H45_RS05705 (1179411) 1179411..1180322 - 912 WP_000705849.1 2-dehydropantoate 2-reductase -
N1H45_RS05710 (1180490) 1180490..1180981 + 492 WP_001138904.1 nucleotide binding protein YajQ -
N1H45_RS05715 (1181109) 1181109..1182473 - 1365 WP_001000978.1 MFS transporter -
N1H45_RS05720 (1182881) 1182881..1183805 + 925 Protein_1123 sel1 repeat family protein -
N1H45_RS05725 (1183861) 1183861..1184403 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
N1H45_RS05730 (1184387) 1184387..1184698 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
N1H45_RS05735 (1184883) 1184883..1185773 - 891 WP_000971336.1 heme o synthase -
N1H45_RS05740 (1185785) 1185785..1186114 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
N1H45_RS05745 (1186114) 1186114..1186728 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
N1H45_RS05750 (1186718) 1186718..1188709 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
N1H45_RS05755 (1188731) 1188731..1189678 - 948 WP_001239440.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T257396 WP_000227784.1 NZ_CP104206:c1184403-1183861 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT257396 WP_001297137.1 NZ_CP104206:c1184698-1184387 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References