Detailed information of TA system TA025737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 74231..74832
• Replicon: plasmid AZO_p6
• Accession: NC_016588
• Organism: Azospirillum lipoferum 4B

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G7ZIL4
• Locus tag: AZOLI_RS29365
• Protein ID: WP_014189911.1
• Coordinates: 74455..74832 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G7ZIZ1
• Locus tag: AZOLI_RS29360
• Protein ID: WP_014189910.1
• Coordinates: 74231..74458 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZOLI_RS29355	71862..74096	-	2235	WP_014189909.1	peptidase	-
AZOLI_RS29360	74231..74458	+	228	WP_014189910.1	antitoxin MazE family protein	Antitoxin
AZOLI_RS29365	74455..74832	+	378	WP_014189911.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AZOLI_RS29370	74870..76324	-	1455	WP_014189912.1	HlyD family efflux transporter periplasmic adaptor subunit	-
AZOLI_RS29375	76321..77265	-	945	WP_065814251.1	efflux RND transporter periplasmic adaptor subunit	-
AZOLI_RS29380	77190..79403	-	2214	WP_162488535.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..295744	295744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-107)

Antitoxin

• Antitoxin MazE-like (9-66)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 12990.88 Da
• Isoelectric Point: 8.0808

>T25737 WP_014189911.1 NC_016588:74455-74832 [Azospirillum lipoferum 4B]
MKRGEIWTVAGGADHAGKPRPVVILQDDRFSGTASVTVCPFTTDPTDAPLFRRPVQPTGGNGLSQSSRIMADKIMTVPRA
KVGHRIGVLEAAHLAWIGKAVLVFLGLAGGGFSEEGAESDPALAG

Nucleotide

• Length: 378 bp

>T25737 NC_016588:74455-74832 [Azospirillum lipoferum 4B]
ATGAAGCGGGGCGAGATCTGGACGGTGGCCGGCGGGGCCGACCATGCGGGAAAGCCGCGGCCGGTCGTCATCCTGCAGGA
CGACCGCTTTTCCGGCACCGCCTCGGTCACCGTCTGTCCGTTCACGACCGATCCGACCGACGCGCCGCTGTTCCGCCGGC
CGGTCCAGCCGACCGGCGGCAACGGCCTGAGCCAGTCCTCCCGCATCATGGCGGACAAGATCATGACGGTTCCGCGGGCC
AAGGTCGGGCATCGGATCGGCGTGCTCGAAGCCGCCCATCTGGCCTGGATCGGCAAGGCCGTCCTCGTGTTTCTCGGTCT
GGCCGGAGGCGGCTTTTCCGAAGAAGGGGCGGAATCCGATCCGGCTCTTGCCGGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8310.33 Da
• Isoelectric Point: 10.6723

>AT25737 WP_014189910.1 NC_016588:74231-74458 [Azospirillum lipoferum 4B]
MPAIRQTSREKVKAHRARLRAQGLRPVQIWVPDVRSPSFVAEARRQSLAVARSPQEADDQGFVDAAGWASLDDGE

Nucleotide

• Length: 228 bp

>AT25737 NC_016588:74231-74458 [Azospirillum lipoferum 4B]
ATGCCTGCAATCCGCCAGACCTCGCGCGAAAAGGTGAAGGCGCACCGTGCGCGGCTTCGGGCGCAGGGGCTGCGTCCGGT
GCAGATTTGGGTCCCCGACGTCCGCAGCCCGTCCTTCGTCGCCGAAGCCCGGCGCCAGTCCCTGGCCGTCGCCCGCAGCC
CGCAGGAGGCCGACGACCAGGGCTTCGTCGATGCCGCGGGCTGGGCTTCGCTGGATGACGGCGAATGA

Structures

Toxin

• AlphaFold DB: G7ZIL4

Antitoxin

• AlphaFold DB: G7ZIZ1