Detailed information of TA system TA025736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 149769..150430
• Replicon: plasmid AZO_p4
• Accession: NC_016587
• Organism: Azospirillum lipoferum 4B

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AZOLI_RS24940
• Protein ID: WP_014189408.1
• Coordinates: 149769..150179 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G7ZGV8
• Locus tag: AZOLI_RS24945
• Protein ID: WP_014189409.1
• Coordinates: 150179..150430 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZOLI_RS24920	145705..146643	-	939	WP_014189403.1	glycosyltransferase family 9 protein	-
AZOLI_RS24925	146640..147953	-	1314	WP_162488422.1	O-antigen ligase family protein	-
AZOLI_RS24930	148358..149242	+	885	WP_014189406.1	GNAT family N-acetyltransferase	-
AZOLI_RS24935	149278..149772	-	495	WP_014189407.1	GNAT family N-acetyltransferase	-
AZOLI_RS24940	149769..150179	-	411	WP_014189408.1	type II toxin-antitoxin system VapC family toxin	Toxin
AZOLI_RS24945	150179..150430	-	252	WP_014189409.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AZOLI_RS24950	150521..151399	-	879	WP_044553129.1	LysR family transcriptional regulator	-
AZOLI_RS24955	151450..152487	-	1038	WP_014189411.1	sodium-dependent bicarbonate transport family permease	-
AZOLI_RS24960	152636..152908	-	273	WP_014189412.1	N-acetyltransferase	-
AZOLI_RS24965	152997..153692	-	696	WP_044553313.1	RNA pseudouridine synthase	-
AZOLI_RS24970	154106..154975	+	870	WP_014189414.1	UTP--glucose-1-phosphate uridylyltransferase GalU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..645253	645253

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-123)

Antitoxin

• SpoVT-AbrB domain (15-56)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14531.69 Da
• Isoelectric Point: 5.6665

>T25736 WP_014189408.1 NC_016587:c150179-149769 [Azospirillum lipoferum 4B]
MLYLLDTNAMSDLIRNPAGPVAGHVRRVGEDAICTSIVVAAELRYGAAKKASAKLSQRVEALLADVAVLPLEPPADAEYG
NIRAELETAGHPIGGNDLLIAAHARTLGVILVTANVREFQRVRGLAVENWLESRPE

Nucleotide

• Length: 411 bp

>T25736 NC_016587:c150179-149769 [Azospirillum lipoferum 4B]
ATGCTCTACCTGCTCGACACCAACGCCATGAGCGACCTGATCCGCAATCCGGCCGGCCCGGTGGCCGGGCATGTGCGGCG
TGTCGGCGAGGATGCGATCTGCACCAGCATCGTGGTGGCTGCGGAATTGCGATACGGCGCCGCGAAGAAGGCATCGGCCA
AGCTGTCGCAGAGGGTCGAGGCGTTGCTGGCCGACGTCGCCGTGCTTCCGCTCGAACCGCCGGCCGATGCCGAATACGGT
AATATCCGGGCGGAGTTGGAGACGGCCGGCCATCCTATCGGCGGCAACGACCTGCTGATCGCCGCCCATGCCCGGACGCT
CGGCGTCATCCTGGTGACGGCGAATGTGCGAGAGTTTCAGCGCGTCCGGGGACTGGCCGTGGAAAACTGGCTGGAAAGCC
GGCCGGAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9624.00 Da
• Isoelectric Point: 4.6437

>AT25736 WP_014189409.1 NC_016587:c150430-150179 [Azospirillum lipoferum 4B]
MPQPATPQRRQVRLFRNNRSQAVRIPAEFELPGESVFISRDGDRLIIEPIRKKGLLALLESWEPLEEEFPDIDDQPVAPK
DIF

Nucleotide

• Length: 252 bp

>AT25736 NC_016587:c150430-150179 [Azospirillum lipoferum 4B]
ATGCCACAGCCAGCGACGCCGCAGCGCCGGCAAGTCCGCCTGTTCCGCAACAACCGCAGCCAGGCCGTCCGCATTCCCGC
CGAATTCGAGCTTCCCGGCGAGTCCGTCTTCATCAGCCGCGACGGCGACCGGCTGATCATCGAACCGATCCGCAAGAAGG
GGCTTCTGGCACTGCTCGAGAGCTGGGAGCCGCTGGAGGAGGAATTCCCCGATATCGACGATCAGCCGGTCGCGCCGAAG
GACATTTTCTGA

Structures

Antitoxin

• AlphaFold DB: G7ZGV8