Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 23935..24578 Replicon plasmid pEcWSU1_A
Accession NC_016515
Organism Enterobacter cloacae EcWSU1

Toxin (Protein)


Gene name vagD Uniprot ID G8LQB2
Locus tag ECWSU1_RS22745 Protein ID WP_014172334.1
Coordinates 24162..24578 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID G8LQB1
Locus tag ECWSU1_RS22740 Protein ID WP_007853351.1
Coordinates 23935..24165 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECWSU1_RS22720 19494..20102 - 609 WP_014172328.1 hypothetical protein -
ECWSU1_RS22725 20147..20503 - 357 WP_041163004.1 hypothetical protein -
ECWSU1_RS22730 21174..21659 + 486 WP_041163005.1 hypothetical protein -
ECWSU1_RS22735 21670..23673 + 2004 WP_014172333.1 AAA family ATPase -
ECWSU1_RS22740 23935..24165 + 231 WP_007853351.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
ECWSU1_RS22745 24162..24578 + 417 WP_014172334.1 type II toxin-antitoxin system VapC family toxin Toxin
ECWSU1_RS22750 24619..25497 - 879 WP_041163006.1 restriction endonuclease -
ECWSU1_RS23795 25857..26440 - 584 Protein_29 LysR family transcriptional regulator -
ECWSU1_RS22760 26824..27054 + 231 WP_041163025.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
ECWSU1_RS22765 27191..27304 - 114 WP_041163008.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..63653 63653
- flank IS/Tn - - 27191..27304 113


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15171.64 Da        Isoelectric Point: 7.8874

>T25734 WP_014172334.1 NC_016515:24162-24578 [Enterobacter ludwigii]
VNKIYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHMQLVDAFCSRLDAILPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLMLEDWVK

Download         Length: 417 bp

>T25734 NC_016515:24162-24578 [Enterobacter ludwigii]
GTGAACAAGATCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTGCTGAAGCGTCT
GGAGCAGGCGGTTCTGCGCGGTCACCGCATTGTGGTTTCGGCCATCACTTATTCTGAAATGCGATTCGGTGCCACCGGCC
CGAAGGCCTCGCCCCGCCACATGCAGCTGGTTGACGCGTTCTGCTCCCGCCTCGATGCTATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCGACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATTGCGGCCGGCGCGGTACTGGTAACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGGACTGATGCTGG
AAGACTGGGTCAAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8674.77 Da        Isoelectric Point: 4.4692

>AT25734 WP_007853351.1 NC_016515:23935-24165 [Enterobacter ludwigii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVSDEGRVNL

Download         Length: 231 bp

>AT25734 NC_016515:23935-24165 [Enterobacter ludwigii]
ATGCGCACAGTTTCTATTTTCAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTTCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGTTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G8LQB2

Antitoxin

Source ID Structure
AlphaFold DB A0A3A3Z894

References