Detailed information of TA system TA025733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 1918..2657
• Replicon: plasmid pEcWSU1_A
• Accession: NC_016515
• Organism: Enterobacter cloacae EcWSU1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: G8LQ82
• Locus tag: ECWSU1_RS22640
• Protein ID: WP_014172305.1
• Coordinates: 2172..2657 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: W7NLL2
• Locus tag: ECWSU1_RS22635
• Protein ID: WP_014172304.1
• Coordinates: 1918..2184 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECWSU1_RS22630	247..1260	-	1014	WP_014172302.1	replication initiation protein	-
ECWSU1_RS22635	1918..2184	+	267	WP_014172304.1	DUF1778 domain-containing protein	Antitoxin
ECWSU1_RS22640	2172..2657	+	486	WP_014172305.1	GNAT family N-acetyltransferase	Toxin
ECWSU1_RS23960	3450..4085	-	636	WP_131494725.1	hypothetical protein	-
ECWSU1_RS24305	4079..4504	-	426	WP_148264544.1	hypothetical protein	-
ECWSU1_RS22650	4525..5652	-	1128	WP_014172311.1	hypothetical protein	-
ECWSU1_RS22655	5903..6241	-	339	Protein_6	capsid portal protein	-
ECWSU1_RS23775	6377..6610	+	234	Protein_7	helix-turn-helix domain-containing protein	-
ECWSU1_RS22660	6933..7550	-	618	WP_014172313.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..63653	63653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (75-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17496.15 Da
• Isoelectric Point: 9.6039

>T25733 WP_014172305.1 NC_016515:2172-2657 [Enterobacter ludwigii]
VGRVTAPEPLSSSHQMAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHVEATGSLRRNM
PDPIPVIILARLAIDASFRGQGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYLHHGFKASQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T25733 NC_016515:2172-2657 [Enterobacter ludwigii]
GTGGGACGTGTAACAGCACCGGAACCTTTATCCAGCTCACATCAAATGGCTGAGTTCGTAAGCGGGGAGGCCGTTCTCGA
TGACTGGCTTAAACAGCGAGGGTTGAAAAATCAGGCACTTGGTGCTGCCAGAACGTTCGTTGTATGTAAAACAGGCACCA
AACAGGTTGCCGGTTTTTACTCCCTGGCCACCGGCAGCGTTAACCATGTAGAAGCAACGGGAAGTCTCAGGCGCAATATG
CCAGATCCAATCCCGGTAATCATACTTGCTCGACTGGCTATCGATGCCTCATTTCGTGGACAAGGGCTGGGTGCTGATTT
ACTGCACGATGCAGTATTGCGCTGTTATCGTGTGGCTGAAAATATAGGCGTTCGTGCAATTATGGTTCATGCGCTGACAG
AAGAAGCCAAAGGATTTTATCTTCATCATGGTTTTAAGGCATCGCAAACCCAAGAACGGACTCTGTTTCTCAAACTGCCG
CAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10049.45 Da
• Isoelectric Point: 4.4733

>AT25733 WP_014172304.1 NC_016515:1918-2184 [Enterobacter ludwigii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDAAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT25733 NC_016515:1918-2184 [Enterobacter ludwigii]
ATGAAATCAGATGTCCAGCTCAACCTCAGAGCCAAGGAGTCTCAAAGAGCACTTATCGATGCTGCTGCAGAAATCCTTCA
TAAATCACGTACAGACTTTATCCTTGAAATGGCGTGCCAGGCTGCTGAGAATGTGATCCTCGATCGTCGCGTATTCAACT
TTAACGATGAGCAGTATGCTGAGTTTATCGATATGCTCGATGCGCCAGTTGCGGATGACGCTGCCATCAATAAACTCCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: G8LQ82

Antitoxin

• AlphaFold DB: W7NLL2