Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4308898..4309395 Replicon chromosome
Accession NZ_CP104123
Organism Escherichia coli strain USDA-ARS-USMARC-49610

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N1705_RS20805 Protein ID WP_001173343.1
Coordinates 4308898..4309071 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N1705_RS20810 Protein ID WP_000398619.1
Coordinates 4309099..4309395 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1705_RS20795 (4305568) 4305568..4307025 + 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -
N1705_RS20800 (4307262) 4307262..4308779 + 1518 WP_001295089.1 lysine--tRNA ligase -
N1705_RS20805 (4308898) 4308898..4309071 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N1705_RS20810 (4309099) 4309099..4309395 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N1705_RS20815 (4309622) 4309622..4309894 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
N1705_RS20820 (4309906) 4309906..4310136 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
N1705_RS20825 (4310317) 4310317..4311948 + 1632 WP_001216486.1 sensor histidine kinase -
N1705_RS20830 (4311945) 4311945..4312664 + 720 WP_196056889.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T257283 WP_001173343.1 NZ_CP104123:c4309071-4308898 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT257283 WP_000398619.1 NZ_CP104123:c4309395-4309099 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References