Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 998144..998804 Replicon chromosome
Accession NZ_CP104123
Organism Escherichia coli strain USDA-ARS-USMARC-49610

Toxin (Protein)


Gene name cptA Uniprot ID A0A3W5XVE6
Locus tag N1705_RS04930 Protein ID WP_000244778.1
Coordinates 998391..998804 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag N1705_RS04925 Protein ID WP_000354046.1
Coordinates 998144..998410 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1705_RS04900 (993313) 993313..994056 + 744 WP_075826628.1 SDR family oxidoreductase -
N1705_RS04905 (994113) 994113..995546 - 1434 WP_001343615.1 6-phospho-beta-glucosidase BglA -
N1705_RS04910 (995591) 995591..995902 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
N1705_RS04915 (996066) 996066..996725 + 660 WP_000250274.1 hemolysin III family protein -
N1705_RS04920 (996921) 996921..997901 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
N1705_RS04925 (998144) 998144..998410 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
N1705_RS04930 (998391) 998391..998804 + 414 WP_000244778.1 protein YgfX Toxin
N1705_RS04935 (998838) 998838..999359 - 522 WP_001055874.1 flavodoxin FldB -
N1705_RS04940 (999471) 999471..1000367 + 897 WP_000806654.1 site-specific tyrosine recombinase XerD -
N1705_RS04945 (1000391) 1000391..1001101 + 711 WP_000715231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
N1705_RS04950 (1001107) 1001107..1002840 + 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 16275.23 Da        Isoelectric Point: 11.7064

>T257267 WP_000244778.1 NZ_CP104123:998391-998804 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQRSR

Download         Length: 414 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT257267 WP_000354046.1 NZ_CP104123:998144-998410 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3W5XVE6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References