257259

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 64953..65488 Replicon plasmid pEc1-50748
Accession NZ_CP104118
Organism Escherichia coli strain USDA-ARS-USMARC-50748

Toxin (Protein)


Gene name relE Uniprot ID T2HS21
Locus tag N1709_RS24325 Protein ID WP_000222775.1
Coordinates 64953..65240 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T2HSA2
Locus tag N1709_RS24330 Protein ID WP_001139207.1
Coordinates 65237..65488 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1709_RS24305 (N1709_24305) 61268..62272 + 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
N1709_RS24310 (N1709_24310) 62551..62790 - 240 Protein_70 hypothetical protein -
N1709_RS24315 (N1709_24315) 63144..63746 + 603 WP_000517694.1 ProQ/FINO family protein -
N1709_RS24320 (N1709_24320) 64398..64943 - 546 WP_000926984.1 hypothetical protein -
N1709_RS24325 (N1709_24325) 64953..65240 - 288 WP_000222775.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N1709_RS24330 (N1709_24330) 65237..65488 - 252 WP_001139207.1 plasmid stabilization protein Antitoxin
N1709_RS24335 (N1709_24335) 65575..65742 - 168 WP_000841010.1 hypothetical protein -
N1709_RS24340 (N1709_24340) 66437..67309 - 873 WP_000729440.1 incFII family plasmid replication initiator RepA -
N1709_RS24345 (N1709_24345) 68012..68653 + 642 WP_000335846.1 transcription termination/antitermination NusG family protein -
N1709_RS24350 (N1709_24350) 68794..69459 + 666 WP_000154143.1 hypothetical protein -
N1709_RS24355 (N1709_24355) 69726..70346 + 621 WP_000176497.1 DUF1449 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / ant(3'')-Ia / aac(3)-VIa / qacE / sul1 / mph(A) htpB 1..126915 126915


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.17 Da        Isoelectric Point: 10.6261

>T257259 WP_000222775.1 NZ_CP104118:c65240-64953 [Escherichia coli]
MTYTVKFRDDALREWLKLDKSVQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9216.45 Da        Isoelectric Point: 4.0927

>AT257259 WP_001139207.1 NZ_CP104118:c65488-65237 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLD
SYL

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB T2HS21

Antitoxin

Source ID Structure
AlphaFold DB A0A754EQ65

References