257255

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) sanaTA/AbiEii-DUF6088
Location 4303472..4304807 Replicon chromosome
Accession NZ_CP104117
Organism Escherichia coli strain USDA-ARS-USMARC-50748

Toxin (Protein)


Gene name sanaT Uniprot ID F4VMR9
Locus tag N1709_RS21040 Protein ID WP_000342497.1
Coordinates 4303472..4304419 (-) Length 316 a.a.

Antitoxin (Protein)


Gene name sanaA Uniprot ID A0A228Z6F8
Locus tag N1709_RS21045 Protein ID WP_000152739.1
Coordinates 4304412..4304807 (-) Length 132 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1709_RS21010 (4299325) 4299325..4300041 + 717 WP_001295734.1 N-acetylneuraminic acid outer membrane channel NanC -
N1709_RS21015 (4300061) 4300061..4301167 + 1107 WP_001298927.1 N-acetylneuraminate epimerase -
N1709_RS21020 (4301232) 4301232..4302212 + 981 WP_000991446.1 sialate O-acetylesterase -
N1709_RS21025 (4302220) 4302220..4302870 - 651 WP_001037963.1 HNH endonuclease -
N1709_RS21030 (4303007) 4303007..4303150 + 144 Protein_4120 HNH endonuclease -
N1709_RS21035 (4303249) 4303249..4303434 + 186 WP_000066585.1 hypothetical protein -
N1709_RS21040 (4303472) 4303472..4304419 - 948 WP_000342497.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein Toxin
N1709_RS21045 (4304412) 4304412..4304807 - 396 WP_000152739.1 DUF6088 family protein Antitoxin
N1709_RS21050 (4304877) 4304877..4305710 - 834 WP_001192741.1 DUF4942 domain-containing protein -
N1709_RS21055 (4306016) 4306016..4307572 + 1557 WP_000202816.1 type I restriction-modification system subunit M -
N1709_RS21060 (4307569) 4307569..4308735 + 1167 WP_001293040.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - fimE / fimB 4296193..4341641 45448


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Nucleotidyl transferase AbiEii toxin, Type IV TA system

(38-303)

Antitoxin

Protein of unknown function DUF6088

(7-108)


Sequences

Toxin        


Download         Length: 316 a.a.        Molecular weight: 35588.79 Da        Isoelectric Point: 4.9737

>T257255 WP_000342497.1 NZ_CP104117:c4304419-4303472 [Escherichia coli]
MDDLTTLFSEVADALGIESVAIVEKDHYIVELLQLLKPLVFDTHQLVFAGGTALAKSGVALNRMSEDVDIKLVPKDAFQA
LSRTQRKNTRKEIVQAVSSAIASSGVFNFDDDYPKVTRDEYRYNDIPVRYPQRVVQAPCLRPFIRLELMEAKLLEPSELR
DIHSLVTGITGKAAIVSAFPCATIISTQAEKLVAMLRRTAAVVRDVERFDDASLVRHIYDTNCIVETSHVDMSLLVDFVQ
QTIEQDIQRYGNQYPQFCASPVDELKMGLDELANNPLYQMRYQQFVSPMVYGERKITWDEAYSRFRSLALAILNA

Download         Length: 948 bp

Antitoxin


Download         Length: 132 a.a.        Molecular weight: 14605.90 Da        Isoelectric Point: 11.6915

>AT257255 WP_000152739.1 NZ_CP104117:c4304807-4304412 [Escherichia coli]
MTIKARIQSRLKRSKRYVFTRDDFKDIAGYDQVGRVLRDLVREGQLLKVGYGVYTKARRNGITGKVMPAAPGGSSAVIVE
TLERLKVRYRFAGATQAYNSGTSTQIPASPEIKTPRRFKRVLSVGNSRLNG

Download         Length: 396 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L5BAQ6

Antitoxin

Source ID Structure
AlphaFold DB A0A228Z6F8

References