257253

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3679798..3680635 Replicon chromosome
Accession NZ_CP104117
Organism Escherichia coli strain USDA-ARS-USMARC-50748

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag N1709_RS18080 Protein ID WP_000227784.1
Coordinates 3680093..3680635 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag N1709_RS18075 Protein ID WP_001297137.1
Coordinates 3679798..3680109 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1709_RS18050 (3674818) 3674818..3675765 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
N1709_RS18055 (3675787) 3675787..3677778 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
N1709_RS18060 (3677768) 3677768..3678382 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
N1709_RS18065 (3678382) 3678382..3678711 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
N1709_RS18070 (3678723) 3678723..3679613 + 891 WP_000971336.1 heme o synthase -
N1709_RS18075 (3679798) 3679798..3680109 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
N1709_RS18080 (3680093) 3680093..3680635 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
N1709_RS18085 (3680691) 3680691..3681626 - 936 WP_260252263.1 tetratricopeptide repeat protein -
N1709_RS18090 (3682034) 3682034..3683398 + 1365 WP_001000978.1 MFS transporter -
N1709_RS18095 (3683526) 3683526..3684017 - 492 WP_001138904.1 nucleotide binding protein YajQ -
N1709_RS18100 (3684185) 3684185..3685096 + 912 WP_000705847.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T257253 WP_000227784.1 NZ_CP104117:3680093-3680635 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT257253 WP_001297137.1 NZ_CP104117:3679798-3680109 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References