257241

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1079181..1079764 Replicon chromosome
Accession NZ_CP104117
Organism Escherichia coli strain USDA-ARS-USMARC-50748

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag N1709_RS05215 Protein ID WP_000254745.1
Coordinates 1079429..1079764 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag N1709_RS05210 Protein ID WP_000581937.1
Coordinates 1079181..1079429 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1709_RS05200 (1075520) 1075520..1076821 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
N1709_RS05205 (1076869) 1076869..1079103 + 2235 WP_000226815.1 GTP pyrophosphokinase -
N1709_RS05210 (1079181) 1079181..1079429 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
N1709_RS05215 (1079429) 1079429..1079764 + 336 WP_000254745.1 endoribonuclease MazF Toxin
N1709_RS05220 (1079835) 1079835..1080626 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
N1709_RS05225 (1080854) 1080854..1082491 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
N1709_RS05230 (1082579) 1082579..1083877 + 1299 WP_000036723.1 phosphopyruvate hydratase -
N1709_RS05235 (1083933) 1083933..1084295 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T257241 WP_000254745.1 NZ_CP104117:1079429-1079764 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT257241 WP_000581937.1 NZ_CP104117:1079181-1079429 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References