257238

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 368539..369339 Replicon chromosome
Accession NZ_CP104117
Organism Escherichia coli strain USDA-ARS-USMARC-50748

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag N1709_RS01715 Protein ID WP_000342449.1
Coordinates 368812..369339 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag N1709_RS01710 Protein ID WP_001277108.1
Coordinates 368539..368805 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1709_RS01690 (364197) 364197..364865 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
N1709_RS01695 (364858) 364858..365916 + 1059 WP_098944931.1 permease-like cell division protein FtsX -
N1709_RS01700 (366161) 366161..367015 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
N1709_RS01705 (367286) 367286..368389 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
N1709_RS01710 (368539) 368539..368805 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
N1709_RS01715 (368812) 368812..369339 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
N1709_RS01720 (369336) 369336..369719 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
N1709_RS01725 (370143) 370143..371252 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
N1709_RS01730 (371300) 371300..372226 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
N1709_RS01735 (372223) 372223..373500 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
N1709_RS01740 (373497) 373497..374264 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T257238 WP_000342449.1 NZ_CP104117:368812-369339 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT257238 WP_001277108.1 NZ_CP104117:368539-368805 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References