Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1043296..1043916 Replicon chromosome
Accession NC_016514
Organism Enterobacter cloacae EcWSU1

Toxin (Protein)


Gene name Hha Uniprot ID G8LPY2
Locus tag ECWSU1_RS04895 Protein ID WP_014168902.1
Coordinates 1043296..1043514 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID W0BRV6
Locus tag ECWSU1_RS04900 Protein ID WP_020885187.1
Coordinates 1043542..1043916 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECWSU1_RS04865 1039307..1039567 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
ECWSU1_RS04870 1039570..1039710 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
ECWSU1_RS04875 1039707..1040417 - 711 WP_014168897.1 GNAT family N-acetyltransferase -
ECWSU1_RS04880 1040518..1041978 + 1461 WP_014168898.1 PLP-dependent aminotransferase family protein -
ECWSU1_RS04885 1041950..1042417 - 468 WP_020885189.1 extracytoplasmic function sigma factor YlaC -
ECWSU1_RS04890 1042535..1043086 - 552 WP_020885188.1 maltose O-acetyltransferase -
ECWSU1_RS04895 1043296..1043514 - 219 WP_014168902.1 hemolysin expression modulator Hha Toxin
ECWSU1_RS04900 1043542..1043916 - 375 WP_020885187.1 Hha toxicity modulator TomB Antitoxin
ECWSU1_RS04905 1044428..1047574 - 3147 WP_014168904.1 multidrug efflux RND transporter permease subunit -
ECWSU1_RS04910 1047597..1048790 - 1194 WP_032677536.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8654.04 Da        Isoelectric Point: 8.9107

>T25723 WP_014168902.1 NC_016514:c1043514-1043296 [Enterobacter ludwigii]
MSEKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWRFVR

Download         Length: 219 bp

>T25723 NC_016514:c1043514-1043296 [Enterobacter ludwigii]
ATGTCTGAAAAACCACTCACAAAAATCGATTATTTGATGCGTTTGCGACGCTGTCAGTCAATAGACACGCTTGAACGTGT
AATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCGGCTGACCACCGTCTTGCTGAAC
TGACCATGAATAAGCTGTATGACAAAATCCCAACGTCCGTGTGGAGATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14495.31 Da        Isoelectric Point: 4.8886

>AT25723 WP_020885187.1 NC_016514:c1043916-1043542 [Enterobacter ludwigii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFINVSRANPVSLSC

Download         Length: 375 bp

>AT25723 NC_016514:c1043916-1043542 [Enterobacter ludwigii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTAAACGAGTTGATCGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGATGAATACCTG
GATGACACCTTTATGTTGTTCAGCAGCTACGGCATTAATGCGCAAGATTTGCAGAAATGGCGTAAATCGGGCAACCGACT
ATTTCGTTGCTTCATCAACGTGAGCAGAGCTAACCCCGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BM33

Antitoxin

Source ID Structure
AlphaFold DB A0A5C1BX74

References