Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1187932..1188550 Replicon chromosome
Accession NZ_CP104114
Organism Escherichia coli strain USDA-ARS-USMARC-51408

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag N1706_RS05555 Protein ID WP_001291435.1
Coordinates 1187932..1188150 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag N1706_RS05560 Protein ID WP_000344800.1
Coordinates 1188176..1188550 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1706_RS05520 (1183221) 1183221..1183793 + 573 WP_000779831.1 YbaY family lipoprotein -
N1706_RS05525 (1183824) 1183824..1184135 - 312 WP_000409911.1 MGMT family protein -
N1706_RS05535 (1184514) 1184514..1184867 + 354 WP_000878140.1 DUF1428 domain-containing protein -
N1706_RS05540 (1184909) 1184909..1186459 - 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
N1706_RS05545 (1186623) 1186623..1187093 - 471 WP_000136192.1 YlaC family protein -
N1706_RS05550 (1187209) 1187209..1187760 - 552 WP_000102564.1 maltose O-acetyltransferase -
N1706_RS05555 (1187932) 1187932..1188150 - 219 WP_001291435.1 HHA domain-containing protein Toxin
N1706_RS05560 (1188176) 1188176..1188550 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
N1706_RS05565 (1189096) 1189096..1192245 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
N1706_RS05570 (1192268) 1192268..1193461 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T257221 WP_001291435.1 NZ_CP104114:c1188150-1187932 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT257221 WP_000344800.1 NZ_CP104114:c1188550-1188176 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References