Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3658778..3659432 Replicon chromosome
Accession NZ_CP104112
Organism Escherichia coli strain USDA-ARS-USMARC-51688

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag N1707_RS17905 Protein ID WP_000244777.1
Coordinates 3658778..3659185 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag N1707_RS17910 Protein ID WP_000354046.1
Coordinates 3659166..3659432 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1707_RS17885 (3654735) 3654735..3656468 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
N1707_RS17890 (3656474) 3656474..3657184 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
N1707_RS17895 (3657209) 3657209..3658105 - 897 WP_000806639.1 site-specific tyrosine recombinase XerD -
N1707_RS17900 (3658217) 3658217..3658738 + 522 WP_001055874.1 flavodoxin FldB -
N1707_RS17905 (3658778) 3658778..3659185 - 408 WP_000244777.1 protein YgfX Toxin
N1707_RS17910 (3659166) 3659166..3659432 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
N1707_RS17915 (3659675) 3659675..3660655 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
N1707_RS17920 (3660851) 3660851..3661510 - 660 WP_000250274.1 hemolysin III family protein -
N1707_RS17925 (3661674) 3661674..3661985 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
N1707_RS17930 (3662030) 3662030..3663463 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
N1707_RS17935 (3663520) 3663520..3664263 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T257212 WP_000244777.1 NZ_CP104112:c3659185-3658778 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT257212 WP_000354046.1 NZ_CP104112:c3659432-3659166 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References