Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 426480..426977 Replicon chromosome
Accession NZ_CP104112
Organism Escherichia coli strain USDA-ARS-USMARC-51688

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag N1707_RS01980 Protein ID WP_001173343.1
Coordinates 426804..426977 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag N1707_RS01975 Protein ID WP_000398619.1
Coordinates 426480..426776 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1707_RS01955 (423211) 423211..423930 - 720 WP_000611288.1 two-component system response regulator DcuR -
N1707_RS01960 (423927) 423927..425558 - 1632 WP_001216477.1 sensor histidine kinase -
N1707_RS01965 (425739) 425739..425969 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
N1707_RS01970 (425981) 425981..426253 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
N1707_RS01975 (426480) 426480..426776 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
N1707_RS01980 (426804) 426804..426977 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
N1707_RS01985 (427096) 427096..428613 - 1518 WP_001295087.1 lysine--tRNA ligase -
N1707_RS01990 (428850) 428850..430307 - 1458 WP_000856835.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T257193 WP_001173343.1 NZ_CP104112:426804-426977 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT257193 WP_000398619.1 NZ_CP104112:426480-426776 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References