Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5285398..5286023 Replicon chromosome
Accession NZ_CP104110
Organism Klebsiella pneumoniae strain USDA-ARS-USMARC-52099

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag N1708_RS25650 Protein ID WP_002882817.1
Coordinates 5285398..5285781 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag N1708_RS25655 Protein ID WP_004150355.1
Coordinates 5285781..5286023 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1708_RS25635 (5282764) 5282764..5283666 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
N1708_RS25640 (5283663) 5283663..5284298 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
N1708_RS25645 (5284295) 5284295..5285224 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
N1708_RS25650 (5285398) 5285398..5285781 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
N1708_RS25655 (5285781) 5285781..5286023 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
N1708_RS25660 (5286228) 5286228..5287145 + 918 WP_080851918.1 alpha/beta hydrolase -
N1708_RS25665 (5287159) 5287159..5288100 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
N1708_RS25670 (5288145) 5288145..5288582 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
N1708_RS25675 (5288579) 5288579..5289439 - 861 WP_004146232.1 virulence factor BrkB family protein -
N1708_RS25680 (5289433) 5289433..5290032 - 600 WP_064167924.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T257192 WP_002882817.1 NZ_CP104110:c5285781-5285398 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT257192 WP_004150355.1 NZ_CP104110:c5286023-5285781 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References