Detailed information of TA system TA257180

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 383155..383741
• Replicon: chromosome
• Accession: NZ_CP104110
• Organism: Klebsiella pneumoniae strain USDA-ARS-USMARC-52099

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: N1708_RS01780
• Protein ID: WP_002920800.1
• Coordinates: 383373..383741 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: N1708_RS01775
• Protein ID: WP_004174006.1
• Coordinates: 383155..383376 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
N1708_RS01755 (379312)	379312..380238	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
N1708_RS01760 (380235)	380235..381512	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
N1708_RS01765 (381509)	381509..382276	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
N1708_RS01770 (382278)	382278..382991	+	714	WP_009484078.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
N1708_RS01775 (383155)	383155..383376	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
N1708_RS01780 (383373)	383373..383741	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
N1708_RS01785 (384014)	384014..385330	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
N1708_RS01790 (385437)	385437..386324	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
N1708_RS01795 (386321)	386321..387166	+	846	WP_004174009.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
N1708_RS01800 (387168)	387168..388238	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	380235..391766	11531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T257180 WP_002920800.1 NZ_CP104110:383373-383741 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T257180 NZ_CP104110:383373-383741 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT257180 WP_004174006.1 NZ_CP104110:383155-383376 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT257180 NZ_CP104110:383155-383376 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A5E5YJY7