Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1379056..1379972 Replicon chromosome
Accession NZ_CP104097
Organism Bacillus subtilis strain GL-4

Toxin (Protein)


Gene name spoIISA Uniprot ID O34853
Locus tag N1207_RS07320 Protein ID WP_003244695.1
Coordinates 1379226..1379972 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID G4EXD8
Locus tag N1207_RS07315 Protein ID WP_003232646.1
Coordinates 1379056..1379226 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1207_RS07280 (1375919) 1375919..1376248 + 330 WP_041850928.1 XkdW family protein -
N1207_RS07285 (1376245) 1376245..1376409 + 165 WP_041850927.1 XkdX family protein -
N1207_RS07290 (1376453) 1376453..1377292 + 840 WP_041850926.1 phage-like element PBSX protein XepA -
N1207_RS07295 (1377345) 1377345..1377614 + 270 WP_003232655.1 hemolysin XhlA family protein -
N1207_RS07300 (1377627) 1377627..1377890 + 264 WP_003232653.1 phage holin -
N1207_RS07305 (1377903) 1377903..1378796 + 894 WP_041850925.1 N-acetylmuramoyl-L-alanine amidase -
N1207_RS07310 (1378833) 1378833..1378970 - 138 WP_003232648.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISC -
N1207_RS07315 (1379056) 1379056..1379226 - 171 WP_003232646.1 three component toxin-antitoxin-antitoxin system antitoxin SpoIISB Antitoxin
N1207_RS07320 (1379226) 1379226..1379972 - 747 WP_003244695.1 toxin-antitoxin-antitoxin system toxin SpoIISA Toxin
N1207_RS07325 (1380082) 1380082..1381083 - 1002 WP_003232642.1 inorganic phosphate transporter -
N1207_RS07330 (1381096) 1381096..1381713 - 618 WP_003218470.1 DUF47 domain-containing protein -
N1207_RS07335 (1381989) 1381989..1383305 - 1317 WP_041850924.1 serine/threonine exchanger -
N1207_RS07340 (1383693) 1383693..1384643 + 951 WP_041344800.1 ring-cleaving dioxygenase -
N1207_RS07345 (1384744) 1384744..1384890 + 147 WP_120363335.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1345183..1400472 55289


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-238)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29061.50 Da        Isoelectric Point: 4.6191

>T257151 WP_003244695.1 NZ_CP104097:c1379972-1379226 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Download         Length: 747 bp

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6654.72 Da        Isoelectric Point: 11.0203

>AT257151 WP_003232646.1 NZ_CP104097:c1379226-1379056 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Download         Length: 171 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3O6Q

Antitoxin

Source ID Structure
PDB 3O6Q
AlphaFold DB G4EXD8

References