Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 509962..510598 Replicon chromosome
Accession NZ_CP104097
Organism Bacillus subtilis strain GL-4

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag N1207_RS02600 Protein ID WP_003156187.1
Coordinates 510248..510598 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag N1207_RS02595 Protein ID WP_003225183.1
Coordinates 509962..510243 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N1207_RS02575 (506322) 506322..506921 - 600 WP_041850724.1 rhomboid family intramembrane serine protease -
N1207_RS02580 (507016) 507016..507381 + 366 WP_015252768.1 holo-ACP synthase -
N1207_RS02585 (507547) 507547..508563 + 1017 WP_072557167.1 outer membrane lipoprotein carrier protein LolA -
N1207_RS02590 (508677) 508677..509846 + 1170 WP_015252766.1 alanine racemase -
N1207_RS02595 (509962) 509962..510243 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
N1207_RS02600 (510248) 510248..510598 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
N1207_RS02605 (510713) 510713..511537 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
N1207_RS02610 (511542) 511542..511907 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
N1207_RS02615 (511911) 511911..512312 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
N1207_RS02620 (512324) 512324..513331 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
N1207_RS02625 (513393) 513393..513722 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
N1207_RS02630 (513719) 513719..514201 + 483 WP_003234299.1 anti-sigma B factor RsbW -
N1207_RS02635 (514167) 514167..514955 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
N1207_RS02640 (514955) 514955..515554 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T257150 WP_003156187.1 NZ_CP104097:510248-510598 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT257150 WP_003225183.1 NZ_CP104097:509962-510243 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References