Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 2095..2630 Replicon plasmid pHB42-5
Accession NZ_CP104082
Organism Escherichia coli strain HB42

Toxin (Protein)


Gene name relE Uniprot ID A0A0D8WEY8
Locus tag N0M81_RS24655 Protein ID WP_032353555.1
Coordinates 2343..2630 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0D8WHE0
Locus tag N0M81_RS24650 Protein ID WP_045145550.1
Coordinates 2095..2346 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0M81_RS24640 (N0M81_24640) 276..1148 + 873 WP_045145554.1 incFII family plasmid replication initiator RepA -
N0M81_RS24645 (N0M81_24645) 1841..2008 + 168 WP_045145553.1 hypothetical protein -
N0M81_RS24650 (N0M81_24650) 2095..2346 + 252 WP_045145550.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
N0M81_RS24655 (N0M81_24655) 2343..2630 + 288 WP_032353555.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
N0M81_RS24660 (N0M81_24660) 2842..4041 - 1200 WP_000948429.1 IS91 family transposase -
N0M81_RS24665 (N0M81_24665) 4051..4239 - 189 WP_000957857.1 hypothetical protein -
N0M81_RS24670 (N0M81_24670) 4354..4665 - 312 Protein_6 colicin-B -
N0M81_RS24675 (N0M81_24675) 4851..5339 + 489 Protein_7 site-specific integrase -
N0M81_RS24680 (N0M81_24680) 5476..5751 - 276 WP_001536593.1 hypothetical protein -
N0M81_RS24685 (N0M81_24685) 5745..6389 - 645 WP_000633911.1 AAA family ATPase -
N0M81_RS24690 (N0M81_24690) 6618..7589 + 972 WP_021580010.1 plasmid segregation protein ParM -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..93173 93173
- flank IS/Tn - - 2842..4041 1199


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11114.16 Da        Isoelectric Point: 10.6261

>T257148 WP_032353555.1 NZ_CP104082:2343-2630 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKAIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSHVYNLASERMR

Download         Length: 288 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9219.48 Da        Isoelectric Point: 4.1789

>AT257148 WP_045145550.1 NZ_CP104082:2095-2346 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHYVDLD
SYL

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WEY8

Antitoxin

Source ID Structure
AlphaFold DB A0A0D8WHE0

References