Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4492324..4492940 Replicon chromosome
Accession NZ_CP104077
Organism Escherichia coli strain HB42

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag N0M81_RS21425 Protein ID WP_001129490.1
Coordinates 4492324..4492698 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag N0M81_RS21430 Protein ID WP_001275523.1
Coordinates 4492698..4492940 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0M81_RS21410 (4489827) 4489827..4490729 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
N0M81_RS21415 (4490726) 4490726..4491361 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
N0M81_RS21420 (4491358) 4491358..4492287 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
N0M81_RS21425 (4492324) 4492324..4492698 - 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
N0M81_RS21430 (4492698) 4492698..4492940 - 243 WP_001275523.1 CopG family transcriptional regulator Antitoxin
N0M81_RS21435 (4493160) 4493160..4493378 - 219 WP_001251290.1 CopG family transcriptional regulator -
N0M81_RS21440 (4494052) 4494052..4494966 - 915 WP_072649161.1 transposase -
N0M81_RS21445 (4494979) 4494979..4495866 - 888 Protein_4188 hypothetical protein -
N0M81_RS21450 (4496282) 4496282..4497223 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
N0M81_RS21455 (4497268) 4497268..4497705 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T257134 WP_001129490.1 NZ_CP104077:c4492698-4492324 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT257134 WP_001275523.1 NZ_CP104077:c4492940-4492698 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References