Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3967352..3967928 Replicon chromosome
Accession NZ_CP104077
Organism Escherichia coli strain HB42

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag N0M81_RS18990 Protein ID WP_001131965.1
Coordinates 3967641..3967928 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag N0M81_RS18985 Protein ID WP_000063158.1
Coordinates 3967352..3967654 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0M81_RS18965 (3962460) 3962460..3963680 + 1221 WP_000873756.1 amino acid deaminase -
N0M81_RS18970 (3963948) 3963948..3966098 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
N0M81_RS18975 (3966148) 3966148..3966351 + 204 WP_000467859.1 YbdD/YjiX family protein -
N0M81_RS18980 (3966362) 3966362..3967318 + 957 WP_001361361.1 GTPase -
N0M81_RS18985 (3967352) 3967352..3967654 - 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
N0M81_RS18990 (3967641) 3967641..3967928 - 288 WP_001131965.1 BrnT family toxin Toxin
N0M81_RS18995 (3968125) 3968125..3969291 + 1167 WP_000800823.1 restriction endonuclease -
N0M81_RS19000 (3969336) 3969336..3969509 + 174 Protein_3716 type I restriction-modification system subunit M N-terminal domain-containing protein -
N0M81_RS19005 (3969503) 3969503..3970171 + 669 Protein_3717 DUF3387 domain-containing protein -
N0M81_RS19010 (3970386) 3970386..3970724 + 339 WP_000132633.1 endoribonuclease SymE -
N0M81_RS19015 (3970812) 3970812..3970859 - 48 WP_229017048.1 hypothetical protein -
N0M81_RS19020 (3971107) 3971107..3972519 + 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T257130 WP_001131965.1 NZ_CP104077:c3967928-3967641 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT257130 WP_000063158.1 NZ_CP104077:c3967654-3967352 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References