257129

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3471579..3472416 Replicon chromosome
Accession NZ_CP104077
Organism Escherichia coli strain HB42

Toxin (Protein)


Gene name itaT Uniprot ID A0A1M0D5U3
Locus tag N0M81_RS16600 Protein ID WP_000227782.1
Coordinates 3471874..3472416 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag N0M81_RS16595 Protein ID WP_001353405.1
Coordinates 3471579..3471890 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
N0M81_RS16570 (3466599) 3466599..3467546 + 948 WP_001239437.1 cytochrome o ubiquinol oxidase subunit II -
N0M81_RS16575 (3467568) 3467568..3469559 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
N0M81_RS16580 (3469549) 3469549..3470163 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
N0M81_RS16585 (3470163) 3470163..3470492 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
N0M81_RS16590 (3470504) 3470504..3471394 + 891 WP_000971327.1 heme o synthase -
N0M81_RS16595 (3471579) 3471579..3471890 + 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
N0M81_RS16600 (3471874) 3471874..3472416 + 543 WP_000227782.1 GNAT family N-acetyltransferase Toxin
N0M81_RS16605 (3472472) 3472472..3473316 - 845 Protein_3248 sel1 repeat family protein -
N0M81_RS16610 (3473723) 3473723..3475087 + 1365 WP_001000978.1 MFS transporter -
N0M81_RS16615 (3475215) 3475215..3475706 - 492 WP_001138904.1 nucleotide binding protein YajQ -
N0M81_RS16620 (3475874) 3475874..3476785 + 912 WP_000705841.1 2-dehydropantoate 2-reductase -
N0M81_RS16625 (3476748) 3476748..3477338 + 591 WP_001276320.1 protein deglycase YajL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19957.31 Da        Isoelectric Point: 9.1763

>T257129 WP_000227782.1 NZ_CP104077:3471874-3472416 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDIIYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCNFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT257129 WP_001353405.1 NZ_CP104077:3471579-3471890 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M0D5U3

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References